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Root development of tissue-cultured papaya shoots in several rooting substrates

机译:组织培养的木瓜芽在几种生根基质中的根系发育

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摘要

The influence of the rooting substrate on the root development of papaya in vitro was studied. The rooting of microcuttings started after the swelling of the basal ends. After 4 weeks of culture, the rooting rate was highest in the starch medium at 96%, followed by the agar, rockwool and vermiculite media. In the gellan gum medium, only 8% of the microcuttings rooted. Anatomically, hypertrophy of subepidermal cells and occurrence of cracking in the epidermis were observed in the starch, agar and gellan gum media. On the other hand, no structural abnormalities were detected in roots formed in the vermiculite and rockwool media. Although, the rooting rate was enhanced with increasing concentrations of agar and gellan gum, the abnormal structures still persisted in both media even when higher concentrations were applied.
机译:研究了生根基质对番木瓜离体根系发育的影响。微切口的生根开始于基端膨胀。培养4周后,生根率在淀粉培养基中最高,为96%,其次是琼脂,岩棉和ver石培养基。在结冷胶培养基中,只有8%的微碎屑生根。解剖学上,在淀粉,琼脂和结冷胶培养基中观察到表皮下细胞肥大和表皮开裂。另一方面,在the石和岩棉介质中形成的根中未检测到结构异常。尽管随着琼脂和结冷胶浓度的增加,生根率会提高,但即使使用较高的浓度,异常结构仍会在两种培养基中持续存在。

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