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Microarray probe expression measures, data normalization and statistical validation

机译:微阵列探针表达测量,数据归一化和统计验证

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摘要

DNA microarray technology is a high-throughput method for gaining information on gene function. Microarray technology is based on deposition/synthesis, in an ordered manner, on a solid surface, of thousands of EST sequences/genes/oligonucleotides. Due to the high number of generated datapoints, computational tools are essential in microarray data analysis and mining to grasp knowledge from experimental results. In this review, we will focus on some of the methodologies actually available to define gene expression intensity measures, microarray data normalization, and statistical validation of differential expression.
机译:DNA微阵列技术是获得有关基因功能信息的高通量方法。微阵列技术基于在固体表面上有序地沉积/合成数千种EST序列/基因/寡核苷酸。由于生成的数据点数量众多,计算工具对于微阵列数据分析和挖掘至关重要,以掌握实验结果中的知识。在本文中,我们将重点介绍一些实际可用的方法,这些方法可用于定义基因表达强度测量,微阵列数据归一化和差异表达的统计验证。

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