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The curse of normalization

机译:归一化的诅咒

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摘要

Despite its enormous promise to further our understanding of cellular processes involved in the regulation of gene expression, microarray technology generates data for which statistical pre-processing has become a necessity before any interpretation of data can begin. The process by which we distinguish (and remove) non-biological variation from biological variation is called normalization. With a multitude of experimental designs, techniques and technologies influencing the acquisition of data, numerous approaches to normalization have been proposed in the literature. The purpose of this short review is not to add to the many suggestions that have been made, but to discuss some of the difficulties were encounter when analysing microarray data.
机译:尽管有巨大的希望可以使我们进一步了解调控基因表达的细胞过程,但微阵列技术可以生成数据,在开始对数据进行任何解释之前,必须对其进行统计预处理。我们将非生物变异与生物变异区分开(并去除)的过程称为标准化。随着影响数据采集的大量实验设计,技术和技术,文献中提出了许多标准化方法。这篇简短评论的目的不是增加已经提出的许多建议,而是讨论分析微阵列数据时遇到的一些困难。

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