...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Comparative and functional genomics >Heterologous array analysis in Pinaceae: hybridization of Pinus taeda cDNA arrays with cDNA from needles and embryogenic cultures of P. taeda, P. sylvestris or Picea abies
【24h】

Heterologous array analysis in Pinaceae: hybridization of Pinus taeda cDNA arrays with cDNA from needles and embryogenic cultures of P. taeda, P. sylvestris or Picea abies

机译：松科中的异源阵列分析：taeda taeda cDNA阵列与针状cDNA和taeda，s.vesvestris或Picea abies的胚发生培养物杂交

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Hybridization of labelled cDNA from various cell types with high-density arrays of expressed sequence tags is a powerful technique for investigating gene expression. Few conifer cDNA libraries have been sequenced. Because of the high level of sequence conservation between Pinus and Picea we have investigated the use of arrays from one genus for studies of gene expression in the other. The partial cDNAs from 384 identifiable genes expressed in differentiating xylem of Pinus taeda were printed on nylon membranes in randomized replicates. These were hybridized with labelled cDNA from needles or embryogenic cultures of Pinus taeda, P. sylvestris and Picea abies, and with labelled cDNA from leaves of Nicotiana tabacum. The Spearman correlation of gene expression for pairs of conifer species was high for needles (r~2 = 0.78-0.86), and somewhat lower for embryogenic cultures (r~2 = 0.68-0.83). The correlation of gene expression for tobacco leaves and needles of each of the three conifer species was lower but sufficiently high (r~2 = 0.52-0.63) to suggest that many partial gene sequences are conserved in angiosperms and gymnosperms. Heterologous probing was further used to identify tissue-specific gene expression over species boundaries. To evaluate the significance of differences in gene expression, conventional parametric tests were compared with permutation tests after four methods of normalization. Permutation tests after Z-normalization provide the highest degree of discrimination but may enhance the probability of type I errors. It is concluded that arrays of cDNA from loblolly pine are useful for studies of gene expression in other pines or spruces.
机译：来自各种细胞类型的标记cDNA与高密度表达序列标签阵列的杂交是研究基因表达的强大技术。很少有针叶树cDNA文库被测序。由于松属和云杉属之间的序列保守性很高，我们研究了使用一种属的阵列研究另一属的基因表达。将来自taeda tausa分化木质部的384个可识别基因的部分cDNA打印在尼龙膜上，随机复制。这些与来自针叶松，樟子松和云杉的针状或胚发生培养物的标记的cDNA以及来自烟草的叶片的标记的cDNA杂交。针叶树对成对的基因表达的Spearman相关性在针中较高（r〜2 = 0.78-0.86），而在胚胎发生培养物中则较低（r〜2 = 0.68-0.83）。三种针叶树种的烟叶和针叶的基因表达相关性较低，但足够高（r〜2 = 0.52-0.63），表明被子植物和裸子植物中有许多部分基因序列是保守的。异源探测进一步用于鉴定物种边界上的组织特异性基因表达。为了评估基因表达差异的重要性，将常规参数测试与四种标准化方法后的置换测试进行了比较。 Z归一化后的置换测试可提供最高的区分度，但可能会增加I型错误的可能性。可以得出结论，火炬松的cDNA阵列可用于研究其他松树或云杉中的基因表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号