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首页> 外文期刊>Comparative biochemistry and physiology, Part C. Pharmacology, toxicology and endocrinology: An international journal >Metallothioneins in liver of Rutilus rutilus exposed to Cu2+. Analysis by metal summation, SH determination and spectrofluorimetry
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Metallothioneins in liver of Rutilus rutilus exposed to Cu2+. Analysis by metal summation, SH determination and spectrofluorimetry

机译：暴露于Cu2 +的Rutilus rut​​ilus肝脏中的金属硫蛋白。金属合计分析，SH测定和荧光分光光度法

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Metallothioneins (MTs) have important roles in the homeostasis of essential metals and in the detoxication of heavy metals. They also represent a potential indicator of aquatic contamination by metals. Routine methods are needed for MTs quantification in ecotoxicological studies. This paper investigates the possibility to use the spectrofluorescent properties of Cu-MTs for MTs quantification. Cu displacement of metals coordinated to MTs and spectrofluorimetric determination of the obtained Cu-MTs was tested with commercial MTs and Cu2+ -induced MTs in roach liver (Rutilus rutilus). Results of this original and simple spectrofluorimetric quantification of MTs presented a good correlation with data obtained with SH quantification, but not with metal summation evaluation of MTs (analysis of Zn, Cu and Cd coordinated to MTs), The three methods showed an clear induction of MTs in roach liver after 7 days of Cu2+ exposure. After 14 days of contamination, a reduction of hepatic MTs content was observable and not correlated to liver recovery. Results show that this low cost spectrofluorimetric method is useful to quantify MTs. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 35]
机译：金属硫蛋白（MTs）在必需金属的稳态和重金属的脱毒中具有重要作用。它们还代表了金属对水生污染的潜在指示。在生态毒理学研究中需要常规方法进行MT定量。本文研究了使用Cu-MT的荧光光谱特性进行MT定量的可能性。用商品MTs和Cu2 +诱导的蟑螂（Rutilus rut​​ilus）测试了与MTs配位的金属的Cu置换和荧光光谱法测定所得的Cu-MTs。最初和简单的MT荧光光谱定量分析结果与通过SH定量获得的数据具有良好的相关性，但与MT的金属合计评估（对MT进行Zn，Cu和Cd的分析）没有很好的相关性，这三种方法显示出明显的Cu2 +暴露7天后蟑螂中的MTs。污染14天后，观察到肝MTs含量降低，并且与肝脏恢复无关。结果表明，这种低成本的荧光光谱法可用于定量MT。 （C）2000 Elsevier Science Inc.保留所有权利。 [参考：35]

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