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首页> 外文期刊>感染症学雑誌 >Evaluation of rapid diagnostic kits for the detection of group A streptococcus to Streptococcus pyogenes and Streptococcus spp. with Lancefield's group A antigen
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Evaluation of rapid diagnostic kits for the detection of group A streptococcus to Streptococcus pyogenes and Streptococcus spp. with Lancefield's group A antigen

机译:评估快速诊断试剂盒,以检测化脓性链球菌和链球菌属的A组链球菌。与兰斯菲尔德的A组抗原

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摘要

We studied the basic performance of eight rapid diagnostic kits for the detection of Group A streptococcus by immunochromatography under the same conditions. Kits were the; QuickVue Dipstick Strep A (Sumitomo Seiyaku Biomedical Co., Ltd.), TESTPACK Plus STREP A (ABBOTT JAPAN Co., Ltd), CLEAVIEW STREP A (Nihon Schering K. K.), QuickVue STREP A (Wako Pure Chemical Industries, Ltd), ImmunoCard STAT! STREP A (TFB, INC.), DIPSTICK 'Eiken' STREP A (Eiken Chemical Co., Ltd.), Rapid Testa Strep A (Daiichi Pure Chemical Co., Ltd.), and StatCheck Strep A (KAINOS Laboratories, Inc.). Four of these kits, i.e. QuickVue Dipstick Strep A, TESTPACK Plus STREP A, Rapid Testa Strep A, and StatCheck Strep A showed sensitivity at 1.0 x 10(5) CFU/mL (1.0 x 10(4)CFU/test) with all of S. pyogenes tested, while the Anginosus group and S. dysgalactiae subsp. equisimilis with Lancefield' s group A antigen showed sensitivity very similar to S. pyogenes. Of these strains, S. dysgalactiae subsp. equisimilis formed a beta-hemolyticcolony resembling that of S. pyogenes on sheep blood agar, and was sensitive to bacitracin. It is thus indispensable to identify the colony using biochemical tests such as the PYR (pyrrolidonylarylamidase production) test. In using rapid diagnostic kits for the detection of Group A streptococcus, it is important to rule out the possibility of Group A streptococcus other than S. pyogenes in throats. Severe invasive group-G streptococcal infections are increasing recently. Concerning S. dysgalactiae subsp. equisimilis, it is especially important to conduct these identification tests.
机译:我们研究了在相同条件下通过免疫色谱法检测A组链球菌的八种快速诊断试剂盒的基本性能。套件是; QuickVue量油尺Strep A(住友制药工业株式会社),TESTPACK Plus STREP A(ABBOTT JAPAN公司),CLEAVIEW STREP A(日本先灵公司​​),QuickVue STREP A(和光纯药工业有限公司),ImmunoCard STAT! STREP A(TFB,INC。),DIPSTICK'Eiken'STREP A(Eiken Chemical Co.,Ltd。),Rapid Testa Strep A(Daiichi Pure Chemical Co.,Ltd.)和StatCheck Strep A(KAINOS Laboratories,Inc.) )。其中的四个套件,即QuickVue Dipstick Strep A,TESTPACK Plus STREP A,Rapid Testa Strep A和StatCheck Strep A在所有条件下的灵敏度均为1.0 x 10(5)CFU / mL(1.0 x 10(4)CFU / test)。化脓性链球菌的测试,而Anginosus组和S. dysgalactiae亚种。具有兰斯菲尔德A组抗原的马齿sim显示出与化脓性链球菌非常相似的敏感性。在这些菌株中,S.dysgalactiae亚种。 equisimilis在绵羊血琼脂上形成类似于化脓性链球菌的β-溶血菌落,并对杆菌肽敏感。因此,必不可少的是使用生化测试(例如PYR(吡咯烷基芳基酰胺酶生产)测试)来鉴定菌落。在使用快速诊断试剂盒检测A组链球菌时,重要的是排除喉咙中化脓性链球菌以外的A组链球菌的可能性。最近,严重的G组侵入性链球菌感染正在增加。关于S. dysgalactiae亚种。相当,进行这些鉴定测试尤为重要。

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