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首页> 外文期刊>感染症学雑誌 >Isolation of thermostable direct hemolysin producing Vibrio parahaemolyticus from food using screening by PCR in food-borne outbreaks
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Isolation of thermostable direct hemolysin producing Vibrio parahaemolyticus from food using screening by PCR in food-borne outbreaks

机译：通过PCR筛选食源性暴发中的热稳定型直接溶血素产生副溶血性弧菌

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摘要

The producibility of thermostable direct hemolysin (TDH) is the most important pathogenic factor in Vibrio parahaemolyticus. TDH (+) V. parahaemolyticus is usually isolated from patients having V. parahaemolyticus food-borne disease. TDH (+) V. parahaemolyticus is, however, very difficult to isolate from food and environmental samples. In the 5 years from 2000 to 2004 in Tokyo, V. parahaemolyticus was isolated from food samples related to 67 of 227 V parahaemolyticus food-borne outbreaks. In these outbreaks, TDH (+) strains were also tried to isolate using PCR as the screening methods. TDH (+) V. parahaemolyticus strains were able to isolate from enrichment broth in which toxR and tdh genes become positive in PCR. TDH (+) strains of the same serotype with patients were able to be isolated from 23 food samples related to 11 outbreaks (16.4%); 3 outbreaks in 2000, 2 in 2001, 2 in 2002, 1 in 2003, and 3 in 2004. The serotypes of V. parahaemolyticus isolated from food were O3 : K6 (10 samples), O3 : K5 (6 samples), O1 : K25 (4 samples), O3 : K29 (2 samples), O4 : K 8 (1 sample), and O4 : K11 (1 sample). The isolation rate of the TDH (+) strain from enrichment broth differed with samples. In several samples TDH (+) strains were isolated easily only by examining 3 colonies, hence no TDH (+) strains were isolated in spite of the examination of 250 colonies. No correlation was seen between the number of V. parahaemolyticus and the isolation rate of TDH (+) strains in food samples. Screening using PCR is very effective method for isolating TDH (+) V. parahaemolyticus from food samples.
机译：热稳定性直接溶血素（TDH）的生产是副溶血性弧菌最重要的致病因素。 TDH（+）副溶血性弧菌通常从患有副溶血性弧菌食源性疾病的患者中分离。但是，很难从食物和环境样品中分离出TDH（+）V. parahaemolyticus。从2000年到2004年的5年中，在东京，从与227例V副溶血性食源性暴发中的67例相关的食物样本中分离了副溶血性弧菌。在这些暴发中，还尝试使用PCR作为筛选方法来分离TDH（+）菌株。 TDH（+）V. parahaemolyticus菌株能够从PCR中toxR和tdh基因变为阳性的富集肉汤中分离出来。能够从与11次暴发有关的23种食物样本中分离出与患者具有相同血清型的TDH（+）菌株（16.4％）; 2000年爆发3次，2001年发生2次，2002年发生2次，2003年爆发1次，2004年发生3次。从食物中分离出的副溶血性弧菌的血清型为O3：K6（10个样品），O3：K5（6个样品），O1： K25（4个样本），O3：K29（2个样本），O4：K 8（1个样本）和O4：K11（1个样本）。 TDH（+）菌株从富集肉汤中的分离率因样品而异。在几个样本中，仅通过检查3个菌落即可轻松分离TDH（+）菌株，因此尽管检查了250个菌落，却没有分离到TDH（+）菌株。食品样品中副溶血性弧菌的数量与TDH（+）菌株的分离率之间没有相关性。使用PCR进行筛选是从食品样品中分离TDH（+）副溶血性弧菌的非常有效的方法。

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