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首页> 外文期刊>感染症学雑誌 >Evaluation of a new real-time PCR assay kit for quantification of human immunodeficiency virus RNA in plasma
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Evaluation of a new real-time PCR assay kit for quantification of human immunodeficiency virus RNA in plasma

机译：评估用于定量血浆中人类免疫缺陷病毒RNA的新型实时PCR分析试剂盒的评估

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摘要

We evaluated the use of COBAS TaqMan HIV-1 for highly pure system (COBAS TaqMan) recently developed as a second-generation quantification of human immunodeficiency virus type 1 (HIV-1) RNA. The linearity, sensitivity, reproducibility, and effects of possibly interfering materials were examined and results compared to those of AMPLICOR HIV-1 MONITOR Test version 1.5 (AMPLICOR) using 6 virus isolates that were all different subtypes. Excellent linearity was obtained at 1.67 x 10(2)-1.73 x 10(6) copies/mL (r2 = 0.991). Sensitivity was 40 copies/mL at a 100% hit rate. Intraexperimental CVs were 27.4-50.8%, and interexperimental CVs were 29.3-81.5%. Although COBAS TaqMan showed an excellent titer correlation with AMPLICOR (r2 = 0.960), mean titer obtained with COBAS TaqMan was 3.1 times higher than that with AMPLICOR (p = 0.002), and 7.1 times higher in a sample of subtype C, suggesting discrepancies in HIV-1 RNA quantification between the two kits. This point should be noted when AMPLICOR is replaced by COBAS TaqMan. Infectious Diseases
机译：我们评估了COBAS TaqMan HIV-1在最近开发为人类免疫缺陷病毒1型（HIV-1）RNA的第二代定量技术的高纯度系统（COBAS TaqMan）中的使用。检查了线性，敏感性，可重复性以及可能干扰物质的影响，并将结果与​​使用6种均为不同亚型的病毒分离株进行的AMPLICOR HIV-1 MONITOR Test 1.5版（AMPLICOR）进行了比较。以1.67 x 10（2）-1.73 x 10（6）拷贝/ mL（r2 = 0.991）获得极好的线性。灵敏度为40拷贝/ mL，命中率为100％。实验内CV为27.4-50.8％，实验间CV为29.3-81.5％。尽管COBAS TaqMan与AMPLICOR的滴度相关性极佳（r2 = 0.960），但COBAS TaqMan的平均滴度是AMPLICOR的3.1倍（p = 0.002），在C型亚型样品中的平均滴度高7.1倍，表明两种试剂盒之间的HIV-1 RNA定量。当将AMPLICOR替换为COBAS TaqMan时，应注意这一点。传染性疾病

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