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首页> 外文期刊>感染症学雑誌 >Evaluation of rapid identification method for Mycobacterium tuberculosis complex using the immunochromatographic slide test kit
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Evaluation of rapid identification method for Mycobacterium tuberculosis complex using the immunochromatographic slide test kit

机译：免疫层析玻片检测试剂盒评估结核分枝杆菌复合物的快速鉴定方法

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摘要

Capilia TB, a lateral flow immunochromatographic slide test kit for directly identifying Mycobacterium tuberculosis complex (MTC), was evaluated by using culture-positive specimens from Mycobacteria Growth Indicator Tubes (MGIT). Sputum specimens from patients suspected of having tuberculosis were treated with NALC-NaOH and cultivated in MGIT960. Liquid specimens were collected from the positive tubes and directly inoculated with Capilia TB. Liquid specimens were also directly tested with AccuProbe. Of the organisms isolated from the 100 MGIT positive tubes, M. tuberculosis complex was identified in 49 (49%) tubes with Capilia TB and not identified in 51 (51%) with Capilia TB. Mycobacterium avium-intracellulare complex (MAC) was identified in 46 (46%) with AccuProbe MAC and other acid-fast bacteria were identified in 5 (5%) by DNA-DNA hybridization method. There were one tube in which M. tuberculosis complex was detected with Capilia TB and M. tuberculosis complex was not detected with AccuProbe MTC, but no tubes in which M. tuberculosis complex was detected with AccuProbe MTC and M. tuberculosis complex was not detected with Capilia TB. Capilia TB is excellent in sensitivity and specificity and very suitable for rapid diagnosis of tuberculosis and is considered to contribute to public health intervention measures taken for the tuberculosis control in Japan.
机译：通过使用分枝杆菌生长指示剂管（MGIT）的培养阳性标本评估了Capilia TB，这是一种用于直接鉴定结核分枝杆菌复合物（MTC）的侧向流免疫层析玻片检测试剂盒。将疑似患有肺结核的患者的痰标本用NALC-NaOH处理并在MGIT960中培养。从阳性管中收集液体样本，并直接用Capilia TB接种。液体样品也直接用AccuProbe测试。从100个MGIT阳性试管中分离出的生物中，在49例（49％）的毛细血管性结核中检出结核分枝杆菌，而在51例（51％）的毛细血管性结核中未检出。用AccuProbe MAC鉴定出鸟分枝杆菌细胞内复合物（MAC）占46（46％），通过DNA-DNA杂交方法鉴定出5（5％）的其他耐酸细菌。有一根试管中的Capilia TB检出了结核分枝杆菌复合物，而用AccuProbe MTC未检出的结核分枝杆菌复合物，但是用AccuProbe MTC检测到的结核分枝杆菌复合物而未检测到结核分枝杆菌复合物的试管却没有。毛细血管结核。 Capilia TB具有出色的敏感性和特异性，非常适合于结核病的快速诊断，并被认为有助于日本为控制结核而采取的公共卫生干预措施。

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