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A case of leptospirosis diagnosed early by flaB-PCR

机译:flaB-PCR早期诊断出钩端螺旋体病

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We report a case of leptospirosis that was diagnosed in the early stage of infection by using flaB-PCR. The flaB gene of Leptospira was amplified from the DNA extract of the patient's blood at hospital day 1, while leptospiral antibody was negative by the method of microscopic agglutination test and micocapsule agglutination test at that time. Seroconversion occurred at hospital day 6 and Leptospira was isolated from the patient's blood at hospital day 16, demonstrating that flaB-PCR method was very useful in early diagnosis of leptospirosis.
机译:我们报告了一例钩端螺旋体病,通过使用flaB-PCR在感染的早期阶段被诊断出。住院第1天从患者血液的DNA提取物中扩增出钩端螺旋体的flaB基因,当时通过显微镜凝集试验和微胶囊凝集试验的方法,钩端螺旋体抗体呈阴性。在医院第6天发生血清转化,在医院第16天从患者血液中分离出钩端螺旋体,表明flaB-PCR方法在钩端螺旋体病的早期诊断中非常有用。

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