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首页> 外文期刊>Comparative biochemistry and physiology. Toxicology & pharmacology: CBP >Molecular cloning and characterization of an ATP-binding cassette (ABC)transmembrane transporter from the white shrimp Litopenaeus vannamei
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Molecular cloning and characterization of an ATP-binding cassette (ABC)transmembrane transporter from the white shrimp Litopenaeus vannamei

机译：南美白对虾南美白对虾ATP结合盒（ABC）跨膜转运蛋白的分子克隆与表征

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摘要

ATP-binding cassette transmembrane transporters (ABC transporters) have a potential role in drug andxenobiotic resistance. Here, we report for the first time the cloning of an ABC transporter from white shrimpLitopenaeus vannamei (designated LvABCG), along with a study of its phylogenetic relationships, andmeasurements of its expression in different shrimp tissues exposed to cadmium and pH stress (acidic andalkaline conditions). Sequence analysis showed that LvABCG shares many similarities with the white/ ABCtransmembrane transporter, including two conserved regions: a highly conserved ATP-binding cassette(ABC) and transmembrane domain (TMD). Spatial analyses of transcript levels for ABCG in shrimp tissues,using reverse transcript PCR, revealed the highest transcript level in the hepatopancreas, less in the intestineand stomach, and none in the other tissues examined. The ABC transporter mRNA transcript in thehepatopancreas of L vannamei was significantly up-regulated after 1.5 h and 24 h of exposure to alkaline andacidic conditions, respectively. LvABCG was also induced in intestine, but was downregulated in the stomachunder the alkaline treatment. Upon exposure to cadmium (4.25 μmol L~(-1)and 8.5 μmol L~(-1))for 48 h, themRNA expression of LvABCG was up-regulated 4.79-fold (at 6 h) and 2.09-fold (at 12 h) in thehepatopancreas. LvABCG was also induced in the stomach after exposure to 4.25 μmol L~(-1)cadmium, butdownregulated in the stomach and intestine after exposure to 8.5 μmol L~(-1)cadmium. These findingsindicate that LvABCG might play an important role in the physiological changes related to metabolism andcell detoxification that occur when Pacific white shrimp are exposed to cadmium and pH stress.
机译：ATP结合盒式跨膜转运蛋白（ABC转运蛋白）在耐药性和外源性耐药中具有潜在作用。在这里，我们首次报道了从白色虾对虾凡纳滨对虾（命名为LvABCG）的ABC转运蛋白的克隆，以及其系统发生关系的研究以及在暴露于镉和pH胁迫（酸性和碱性条件）的不同虾组织中其表达的测量方法。 ）。序列分析表明，LvABCG与白色/ ABC跨膜转运蛋白有许多相似之处，包括两个保守区域：一个高度保守的ATP结合盒（ABC）和跨膜结构域（TMD）。使用逆转录PCR对虾组织中ABCG的转录水平进行空间分析，结果显示肝胰腺中的转录水平最高，肠和胃中的转录水平最高，而其他检查的组织中没有。暴露于碱性和酸性条件下1.5 h和24 h后，南美白对虾肝胰腺中的ABC转运蛋白mRNA转录显着上调。 LvABCG也可在肠中被诱导，但在碱性处理下在胃中被下调。暴露于镉（4.25μmolL〜（-1）和8.5μmolL〜（-1））48小时后，LvABCG的RNA表达被上调了4.79倍（在6 h时）和2.09倍（在12时） h）在肝胰腺中。 LvABCG暴露于4.25μmolL〜（-1）镉后也会在胃中被诱导，但暴露于8.5μmolL〜（-1）镉后在胃和肠中被下调。这些发现表明，当太平洋白虾暴露于镉和pH胁迫时，LvABCG可能在与代谢和细胞排毒有关的生理变化中起重要作用。

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