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首页> 外文期刊>Comparative biochemistry and physiology, Part C. Pharmacology, toxicology and endocrinology: An international journal >A thyrotropin-like molecule from the pituitary of an Indian freshwater murrel: comparison of its biological activity with other thyrotropins
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A thyrotropin-like molecule from the pituitary of an Indian freshwater murrel: comparison of its biological activity with other thyrotropins

机译：来自印度淡水穆雷尔垂体的促甲状腺素样分子：其生物学活性与其他促甲状腺素的比较

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摘要

Murrel pituitary thyrotropin-like molecule (mTSH) was purified to homogeneity with the help of a convenient and sensitive in vitro assay system where addition of this material to the thyroid follicle incubation stimulated thyroxine (T-4) secretion into the medium. Pituitary extract of a freshwater murrel, Channa punctatus, was solvent extracted to obtain glycoprotein enriched fraction. This was subjected to Sephadex G-100 gel filtration and eluate of void volume (peak I) showed strong TSH activity las reflected from T-4 secretion) which was further purified by using concanavalin A-Sepharose, FPLC Mono Q and immunoaffinity chromatography. Purified mTSH gave a single band in PAGE, and SDS PAGE revealed two dissimilar subunits, alpha and beta. Addition of increasing concentrations of mTSH, Indian carp TSH (cTSH) and bovine TSH (bTSH) to in vitro murrel thyroid follicle incubations caused a linear increase in thyroxine (T-4) release into the medium, effect was highest with mTSH and lowest with bTSH. However. in in vivo experiments, injections of increasing doses of mTSH to murrel elevated plasma T-4 level in a linear manner while bTSH gave a biphasic response. Addition of mTSH and bTSH to rat or goat thyroid epithelial cell incubations equally stimulated T-4 release into the medium, while cTSH had significantly less effect. Binding affinity (K-a) and receptor occupancy (B-max) of mTSH to murrel thyroid follicular membrane preparation was considerably higher in comparison to cTSH or bTSH whereas both mTSH and bTSH had nearly similar K-a and B-max with rat thyroid epithelial cell membrane preparation. Findings indicate that mTSH is a more potent TSH as compared to carp and bovine TSH in murrel and has equipotent biological activity as bTSH on rat and goat thyroid. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 38]
机译：借助于便利和灵敏的体外测定系统，将Murrel垂体促甲状腺素样分子（mTSH）纯化至均质，在该系统中，将这种物质添加到甲状腺滤泡培养中可刺激甲状腺素（T-4）分泌到培养基中。溶剂提取淡水鼠尾草Channa punctatus的垂体提取物，以获得富含糖蛋白的馏分。将其进行Sephadex G-100凝胶过滤，洗脱液的空隙体积（峰I）显示出很强的TSH活性（从T-4分泌反映出来），通过使用伴刀豆球蛋白A-Sepharose，FPLC Mono Q和免疫亲和层析进一步纯化。纯化的mTSH在PAGE中显示一条条带，而SDS PAGE显示两个不同的亚基，α和β。将mTSH，印度鲤TSH（cTSH）和牛TSH（bTSH）的浓度增加到体外Murrel甲状腺滤泡培养中导致向培养基中释放的甲状腺素（T-4）呈线性增加，在mTSH中效果最高，在MTSH中效果最低bTSH。然而。在体内实验中，向murrel注射增加剂量的mTSH以线性方式升高血浆T-4水平，而bTSH则产生双相反应。在大鼠或山羊甲状腺上皮细胞培养物中加入mTSH和bTSH同样会刺激T-4释放到培养基中，而cTSH的作用明显较小。与cTSH或bTSH相比，mTSH与壁栗甲状腺滤泡膜制剂的结合亲和力（Ka）和受体占有率（B-max）明显更高，而mTSH和bTSH与大鼠甲状腺上皮细胞膜制剂的Ka和B-max几乎相似。研究结果表明，与鲤鱼和牛TSH相比，mTSH在鼠尾草中具有更强的促甲状腺激素作用，对大鼠和山羊甲状腺具有与bTSH相同的生物学活性。 （C）2000 Elsevier Science Inc.保留所有权利。 [参考：38]

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