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首页> 外文期刊>Comparative biochemistry and physiology, Part C. Pharmacology, toxicology and endocrinology: An international journal >Effect of the dietary brominated phenol, lanasol, on chemical biotransformation enzymes in the gumboot chiton Cryptochiton stelleri (Middendorf, 1846)
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Effect of the dietary brominated phenol, lanasol, on chemical biotransformation enzymes in the gumboot chiton Cryptochiton stelleri (Middendorf, 1846)

机译：日粮中的溴化苯酚，lamasol对口香糖奇脱体Cryptochiton stelleri中化学生物转化酶的影响（Middendorf，1846年）

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The effects of diet and other non-anthropogenic stressors on biochemical defenses and their relationship to susceptibility have been largely ignored in wildlife populations. Lanosol is a compound found in relatively high amounts in various marine species of Rhodophyta, including Odonthalia dentata. While previous studies demonstrated that lanosol is a feeding deterrent to several marine herbivores, Cryptochiton stelleri readily feeds upon O. dentata. To examine the effects of lanosol on the profile of biochemical defenses in C. stelleri, chitons were gavaged daily with 0, 1, 2.5, 5, or 10 mg/kg of lanosol. After three days of exposure, digestive gland microsomes were probed for expression of homologous isoforms of cytochromes P450 (CYP1A, CYP3A, and CYP2) and phase II enzymatic activities. Expression of a 43 kDa CYP3A-like protein was increased by approximately 45% over control following 2.5, 5, and 10 mg/kg treatments. Estradiol hydroxylase activity tended to increase with the dose of lanosol. UDP-glucuronosyl transferase activity was highly variable but appeared to increase at the two highest treatments, while sulfotranserase activity was significantly decreased at the three highest doses. Kinetic studies of CST activity showed lanosol is a non-competitive inhibitor of both CDNB and GSH in the GST-mediated conjugation reaction. These results show that dietary exposure to the brominated-phenol, lanosol, may alter expression and activity of some phase I and II biotransformation enzymes in chitons, potentially providing a dietary advantage for the species. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 40]
机译：饮食和其他非人为压力源对生化防御的影响及其与易感性的关系在野生动植物种群中已被大大忽略。羊毛脂是在红藻的各种海洋物种中发现的相对较高的化合物，包括齿齿O。先前的研究表明，山no酚是几种海洋食草动物的摄食威慑力，而隐rypto虫则很容易以齿齿稻为食。为了检查羊毛酚对斯特氏梭菌的生化防御特性的影响，每天用0、1、2.5、5或10 mg / kg羊毛酚对Chitons进行灌胃。暴露三天后，对消化腺微粒体进行细胞色素P450同源同工型（CYP1A，CYP3A和CYP2）表达和II期酶活性的检测。在进行2.5、5和10 mg / kg的处理后，与对照相比，43 kDa CYP3A样蛋白的表达增加了约45％。雌二醇羟化酶活性倾向于随剂量的羊毛脂而增加。 UDP-葡糖醛酸糖基转移酶的活性变化很大，但在两个最高剂量的治疗中似乎增加，而在三个最高剂量时磺基转移酶的活性却明显降低。 CST活性的动力学研究表明，羊毛脂在GST介导的偶联反应中是CDNB和GSH的非竞争性抑制剂。这些结果表明，饮食中接触溴化苯酚，羊毛酚可能会改变Chitons中某些I和II期生物转化酶的表达和活性，从而可能为该物种提供饮食优势。 （C）2000 Elsevier Science Inc.保留所有权利。 [参考：40]

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