Immunological significance of metal induced conformational changes in the mitogenic Achatinin(H) binding to carbohydrate ligands

Indra D.; Ramalingam K.; Asokan C.; Jayakumar R.; Ganesh S.

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Immunological significance of metal induced conformational changes in the mitogenic Achatinin(H) binding to carbohydrate ligands

机译：金属诱导有丝分裂的Achatinin（H）与碳水化合物配体结合的构象变化的免疫学意义

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9-O-Acetyl neuraminic acid specific lectin (Achatinin(H)) was isolated from the hemolymph of the land snail Achatina fulica by affinity chromatography on sheep submaxillary mucin (SSM) coupled cyanogen bromide activated Sepharose 4B. The molecular weight of the native protein was 2.42 kDa. UV-Vis absorption, fluorescence and circular dichroism spectroscopic studies on Achatinin(H) revealed the importance of divalent metal ions (Ca2+, Mg2+ and Mn2+) on lectin conformational change associated with activity of lectins. The binding of these cations changes lambda(max) to shorter wavelength in the far UV region (blue shift) and longer wavelength in UV region (red shift), indicating substantial contribution of aromatic side chain in the far UV region on binding with metal ions. The results infer that divalent cations cause conformational changes in lectin which may be responsible for affinity with their carbohydrate moiety. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 27]

机译：通过亲和层析在绵羊下颌粘蛋白（SSM）偶联的溴化氰活化的Sepharose 4B上从田螺Achatina fulica的血淋巴中分离出9-O-乙酰神经氨酸特异性凝集素（Achatinin（H））。天然蛋白质的分子量为2.42kDa。 Achatinin（H）的UV-Vis吸收，荧光和圆二色光谱研究表明，二价金属离子（Ca2 +，Mg2 +和Mn2 +）对于与凝集素活性相关的凝集素构象变化非常重要。这些阳离子的结合将lambda（max）更改为在远紫外区域的较短波长（蓝移）和在紫外区域的较长波长（红移），表明远紫外区域中的芳族侧链对与金属离子的结合起了重要作用。结果推断，二价阳离子引起凝集素的构象变化，这可能导致与其碳水化合物部分的亲和力。（C）2000 Elsevier Science Inc.保留所有权利。 [参考：27]

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《Comparative biochemistry and physiology, Part C. Pharmacology, toxicology and endocrinology: An international journal》 |2000年第2期|共7页
作者
Indra D.; Ramalingam K.; Asokan C.; Jayakumar R.; Ganesh S.;
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正文语种 eng
中图分类内分泌生理;
关键词
Achatinin(h); Circular dichroism; Conformational changes; Hydrophobic environment; Divalent cations; Metal binding specificity; Immunomodulation; Antigenic determinant; Lectin; Domain;

机译：Achatinin（h）;圆形二色性;构象变化;疏水环境;二价阳离子;金属结合特异性;免疫调节;抗原决定簇;凝集素;域;

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1. Immunological significance of metal induced conformational changes in the mitogenic Achatinin(H) binding to carbohydrate ligands [J] . Indra D., Ramalingam K., Asokan C., Comparative biochemistry and physiology, Part C. Pharmacology, toxicology and endocrinology: An international journal . 2000,第2期

机译：金属诱导有丝分裂的Achatinin（H）与碳水化合物配体结合的构象变化的免疫学意义
2. Immunological significance of metal induced conformational changes in the mitogenic Achatinin(H) binding to carbohydrate ligands [J] . Indra D., Ramalingam K., Asokan C., Comparative biochemistry and physiology, Part C. Pharmacology, toxicology and endocrinology: An international journal . 2000,第2期

机译：金属诱导有丝分裂的Achatinin（H）与碳水化合物配体结合的构象变化的免疫学意义
3. Immunological significance of metal induced conformational changes in the mitogenic Achatinin_H bidning to carbohydrate ligands [J] . D.Indra, S.Ganesh, K.Ramaligam, Comparative biochemistry and physiology. Toxicology & pharmacology: CBP . 2000,第2期

机译：金属诱导有丝分裂的Achatina与碳水化合物配体结合的构象变化的免疫学意义
4. Investigation of the CCL5-Chondrotin Sulfate Complex Using Hydroxyl Radical Footprinting Reveals Information Involving Ligand-Binding, Ligand-Induced Conformational Change and pH-Dependent Aggregation [C] . Caroline Watson, Xu Wang, Vitor H. Pomin, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：使用羟基自由基足迹对CCL5-chondrotin硫酸盐复合物的研究显示涉及配体结合，配体诱导的构象变化和pH依赖聚集的信息
5. Experimental and theoretical studies of binding interactions in divalent transition metal cation-N-donor ligand complexes: Structures, sequential bond dissociation energies, mechanisms and energetics of collision-induced dissociation. [D] . Nose, Holliness. 2014

机译：二价过渡金属阳离子-N-供体配体配合物中结合相互作用的实验和理论研究：结构，顺序键解离能，碰撞诱导解离的机理和能量。
6. Conformational changes of the glucocorticoid receptor ligand binding domain induced by ligand and cofactor binding and the location of cofactor binding sites determined by hydrogen/deuterium exchange mass spectrometry [O] . Lee Frego, Walter Davidson 2006

机译：配体和辅因子结合引起的糖皮质激素受体配体结合域的构象变化以及氢/氘交换质谱法测定的辅因子结合位点的位置
7. The use of in vitro peptide binding profiles and in silico ligand-receptor interaction profiles to describe ligand-induced conformations of the retinoid X receptor alpha ligand-binding domain [O] . Folkertsma S., Noort P.I., Heer A., 2007

机译：使用体外肽结合谱和计算机配体-受体相互作用谱描述类视色素X受体α配体结合域的配体诱导构象

Immunological significance of metal induced conformational changes in the mitogenic Achatinin(H) binding to carbohydrate ligands

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