...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Comparative biochemistry and physiology. Toxicology & pharmacology: CBP >Neurotoxicological effects of a primary and ozonated treated wastewater on freshwater mussels exposed to an experimental flow-through system
【24h】

Neurotoxicological effects of a primary and ozonated treated wastewater on freshwater mussels exposed to an experimental flow-through system

机译：初级和臭氧处理过的废水对暴露于实验流通系统的淡水贻贝的神经毒理学影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The neurotoxic potential of a primary-treated and ozonated municipal effluent was examined using feral freshwater Elliptio complanata mussels. Specimens were exposed to increasing concentrations (0, 1, 3, 10 and 20% v/v) of a primary-treated effluent before and after treatment with 10 mg/L of ozone in a mesocosm-type experiment for 30 days. A suite of biomarkers was used to assess the potential neurotoxic stress of the wastewaters on these benthic invertebrates: opiate binding sites, γ-aminobutyric acid (GABA) metabolism, monoamines levels (serotonin, dopamine), monoamine oxidase, acetylcholinesterase and lipid peroxidation. Gametogenic activity was also determined by the gonado-somatic index and by vitellogenin-like proteins. The results show that the number of opiate binding sites increased slightly, especially after ozonation. GABA metabolism was generally reduced, suggesting higher glutamate stimulation than GABA dampening effects in mussel ganglia. This excitatory state was further confirmed by decreased acetylcholinesterase activity in gonadal tissues. The turnover of dopamine was enhanced with increased serotonin levels, but accompanied by reduced catabolism, as evidenced by decreased monoamine oxidase activity. Moreover, oxidative stress was increased, as determined by lipid peroxidation in the gonad (containing ganglia), which was significantly correlated with acetylcholinesterase activity and dopamine metabolism. The gonado-somatic index was significantly reduced with increased levels of vitellogenin-like proteins, again confirming the estrogenic action of these wastewaters. The data suggest that exposure to a primary-treated municipal effluent before and after ozonation leads to an excitotoxic syndrome implicating perturbations in GABA, dopamine and acetylcholine signaling. The increase in dopamine metabolism may be associated with the occurrence of opiate-like compounds (i.e. morphine) in the effluent. In general, ozonation reduced the severity of the responses, indicating that this disinfection strategy does not increase neurotoxicity to mussels.
机译：使用野生淡水Elliptio complanata贻贝检查了经过初步处理和臭氧处理的市政污水的神经毒性潜力。在中膜宇宙型实验中，样品在用10 mg / L臭氧处理之前和之后暴露于浓度升高的浓度（0、1、3、10和20％v / v）的初级处理废水中，持续30天。一套生物标记用于评估废水对这些底栖无脊椎动物的潜在神经毒性压力：鸦片结合位点，γ-氨基丁酸（GABA）代谢，单胺水平（血清素，多巴胺），单胺氧化酶，乙酰胆碱酯酶和脂质过氧化。致配子活性也由性腺体细胞指数和卵黄蛋白原样蛋白确定。结果表明，鸦片结合位点的数量略有增加，特别是在臭氧化之后。在贻贝神经节中，GABA代谢通常会降低，这表明谷氨酸刺激比GABA抑制作用要高。性腺组织中乙酰胆碱酯酶活性的降低进一步证实了这种兴奋状态。多巴胺的转换随着血清素水平的提高而增加，但伴随着分解代谢的降低，这可以通过单胺氧化酶活性的降低来证明。此外，通过性腺（含神经节）中的脂质过氧化确定，氧化应激增加，这与乙酰胆碱酯酶活性和多巴胺代谢显着相关。随着卵黄蛋白原样蛋白水平的增加，性腺体细胞指数显着降低，再次证实了这些废水的雌激素作用。数据表明，臭氧处理之前和之后暴露于经过主要处理的城市污水中会导致兴奋性毒性综合症，从而引起GABA，多巴胺和乙酰胆碱信号传导的扰动。多巴胺代谢的增加可能与废水中阿片样化合物（即吗啡）的发生有关。通常，臭氧处理可降低反应的严重程度，表明这种消毒策略不会增加对贻贝的神经毒性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号