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首页> 外文期刊>藥學雜誌 >Alterations of antiproliferative effects of serum obtained from patients with acute cerebral infarction treated with a radical scavenger, edaravone, with or without amlodipine using an in vitro cultured basilar artery smooth muscle cells
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Alterations of antiproliferative effects of serum obtained from patients with acute cerebral infarction treated with a radical scavenger, edaravone, with or without amlodipine using an in vitro cultured basilar artery smooth muscle cells

机译：使用体外培养的基底动脉平滑肌细胞，用自由基清除剂依达拉奉联合或不联合氨氯地平治疗急性脑梗死患者血清的抗增殖作用变化

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The guinea-pig basilar artery smooth muscle cell (GBa-SM3) culture system in the Dulbecco's modified Eagle's medium for 3 days serves as a useful in vitro model for assessing antiproliferative effects of various therapeutic agents on vessels. With use of this system we studied whether human serum obtained from patients with acute cerebral infarction (n = 16) would have a proliferative effect on vessels and whether an administration of a free radical scavenger, edaravone, with or without amlodipine would elicit antiproliferative effects. The control serum was obtained from 3 healthy human subjects. Time courses of the cell growth and survival were measured colorimetrically by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrzolium bromide (MTT) test. The stimulatory effect on the proliferation of GBa-SM3 cells of patients' serum obtained immediately after infarction was significantly (p < 0.05) greater than those obtained from the same patients after the treatment of edaravone for 2 weeks. In addition,the serum obtained from the patients treated by edaravone and amlodipine (n = 7) showed a significantly (p < 0.05) greater antiproliferative effect than that obtained from those treated by edaravone (n = 9). In conclusion, edaravone may have a clinically beneficial antiproliferative effect on vascular smooth muscle cells. Co-administration of amlodipine, possessing an antioxidative calcium channel blocker, with edaravone may be a promising combination to patients with acute cerebral infarction. Further controlled clinical trials with a large number of patients should be warranted.
机译：豚鼠基底动脉平滑肌细胞（GBa-SM3）培养系统在Dulbecco改良的Eagle培养基中培养3天，可作为一种有用的体外模型，用于评估各种治疗剂对血管的抗增殖作用。通过使用该系统，我们研究了从急性脑梗死患者（n = 16）获得的人血清是否会对血管产生增生作用，以及自由基清除剂依达拉奉与或不与氨氯地平合用是否会产生抗增殖作用。对照血清获自3名健康人受试者。通过3- [4,5-二甲基噻唑-2-基] -2,5-二苯基溴化四氮唑（MTT）比色法测量细胞生长和存活的时程。梗死后立即获得的患者血清对GBa-SM3细胞增殖的刺激作用显着（p <0.05）大于依达拉奉治疗2周后从相同患者中获得的刺激作用。此外，从依达拉奉和氨氯地平治疗的患者（n = 7）获得的血清比从依达拉奉治疗的患者（n = 9）获得显着（p <0.05）更大的抗增殖作用。总之，依达拉奉可能对血管平滑肌细胞具有临床有益的抗增殖作用。具有抗氧化钙通道阻滞剂的氨氯地平与依达拉奉并用可能是急性脑梗死患者的有前途的组合。应当对大量患者进行进一步的对照临床试验。

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