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首页> 外文期刊>Biopreservation and biobanking >Osmotic Damage as a Predictor of Motility Loss During Convective Desiccation of Bovine Sperm
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Osmotic Damage as a Predictor of Motility Loss During Convective Desiccation of Bovine Sperm

机译：渗透性损害作为牛精子对流干燥过程中动力丧失的预测因子

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摘要

Current state-of-the art technologies are lagging in the application of desiccation storage to mammalian cells using nonreducing sugars. For bovine sperm, motility is irreversibly lost before reaching a sufficiently low moisture content necessary for preservation. It is hypothesized that much of the damage during drying is related to the osmotic stress encountered due to increased osmolarity of the extracellular environment. To test this hypothesis, we subjected sperm to liquid hyperosmotic environments for varying time-periods and measured their motility. We then extracted parameters for two models for motility loss based on these experiments: a firstorder rate injury model (Fast or Slow) and a multi-modal (MM) injury model. The MM injury model incorporated an additional function accounting for damage induced by a time-independent osmotic change. Based on these models, we predicted sperm motility loss measured from natural and forced convective desiccation experiments. The MM injury model was able to closely bracket motility loss for desiccation as an osmotic change event with time-independent and time-dependent components. While the mechanistic basis of osmotic damage requires further exploration, the model can serve as a bracketing tool for predicting motility loss during desiccation based on excipients designed to minimize osmotic damage.
机译：当前的最新技术在使用非还原糖将干燥存储应用于哺乳动物细胞方面处于滞后状态。对于牛精子，在达到保存所需的足够低的水分含量之前，运动力就不可逆转地丧失了。据推测，干燥过程中的大部分损害与由于细胞外环境渗透压增加而遇到的渗透压有关。为了验证这一假设，我们将精子置于液体高渗环境中变化了不同的时间，并测量了它们的运动能力。然后，基于这些实验，我们提取了两种动力丧失模型的参数：一阶损伤模型（快或慢）和多模式损伤模型。 MM损伤模型包含一个附加函数，该函数解释了与时间无关的渗透变化引起的损伤。基于这些模型，我们预测了通过自然和强迫对流干燥实验测得的精子活力丧失。 MM损伤模型能够将干燥引起的动力丧失紧密地归结为具有时间独立和时间依赖成分的渗透变化事件。尽管渗透损伤的机理基础需要进一步研究，但该模型可以用作基于干燥剂的渗透预测支架，该赋形剂基于旨在使渗透损伤最小化的赋形剂。

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