• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Comparative Medicine >Effect of Prophylactic Supplementation with Grape Polyphenolics on Endotoxin-Induced Serum Secretory Phospholipase A(2) Activity in Rats
【24h】

Effect of Prophylactic Supplementation with Grape Polyphenolics on Endotoxin-Induced Serum Secretory Phospholipase A(2) Activity in Rats

机译：预防性补充葡萄多酚对内毒素诱导的大鼠血清分泌磷脂酶A（2）活性的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study investigated whether dietary supplementation of polyphenolics-rich grape extract (GE) could attenuate endotoxin-induced serum secretory phospholipase A(2) (sPLA(2)) activity, a modulator of inflammation. Male Sprague Dawley rats were fed a control diet or the diet supplemented with polyphenolic-rich GE (100 or 300 mg/kg daily) for 3 wk prior to intraperitoneal injection of 3 or 15 mg/kg LPS. A fluorometric assay was used to measure serum sPLA(2) activity during a 5-d period before and after LPS injection. Body weight, hematocrit, and serum C-reactive protein level were also measured. Administration of LPS induced a rapid increase in sPLA(2) activity, which peaked 1 to 2 d after LPS injection and resolved to near-baseline values on days 4 to 5. Marked declines in body weight and hematocrit, increases in C-reactive protein levels, and effects on health status also occurred. GE supplementation significantly attenuated the LPS-induced increase in sPLA(2) activity and decline in hematocrit, but its effects on the loss of body weight and C-reactive protein levels were not significant. Among the measurements, serum sPLA(2) was the only marker that showed a dose-dependent response to both LPS and GE supplementation. The current findings show that oral consumption of polyphenolic-rich GE suppresses endotoxin-induced sPLA(2) activity.
机译：这项研究调查饮食是否补充富含多酚的葡萄提取物（GE）是否可以减弱内毒素诱导的血清分泌性磷脂酶A（2）（sPLA（2））活性，它是炎症的调节剂。在腹膜内注射3或15 mg / kg LPS之前，先给雄性Sprague Dawley大鼠喂食对照饮食或补充富含多酚的GE（每天100或300 mg / kg）的饮食3周。在注入LPS之前和之后的5 d内，使用荧光测定法测量血清sPLA（2）活性。还测量了体重，血细胞比容和血清C反应蛋白水平。 LPS的使用引起sPLA（2）活性的快速增加，在LPS注射后1到2 d达到峰值，并在4到5 d达到接近基线的值。体重和血细胞比容明显下降，C反应蛋白增加水平，以及对健康状况的影响。 GE补充剂显着减弱LPS诱导的sPLA（2）活性增加和血细胞比容下降，但对体重减轻和C反应蛋白水平的影响并不明显。在这些测量中，血清sPLA（2）是唯一显示对LPS和GE剂量均呈剂量依赖性反应的标志物。当前的发现表明，口服富含多酚的GE可抑制内毒素诱导的sPLA（2）活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号