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首页> 外文期刊>Comparative Medicine >Multispecies Epidemiologic Surveillance Study after an Outbreak of Yersiniosis at an African Green Monkey Research Facility
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Multispecies Epidemiologic Surveillance Study after an Outbreak of Yersiniosis at an African Green Monkey Research Facility

机译：非洲绿猴研究设施耶尔森病爆发后的多物种流行病学监测研究

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After an outbreak of Yersinia enterocolitica at a NHP research facility, we performed a multispecies investigation of the prevalence of Yersinia spp. in various mammals that resided or foraged on the grounds of the facility, to better understand the epizootiology of yersiniosis. Blood samples and fecal and rectal swabs were obtained from 105 captive African green monkeys (AGM), 12 feral cats, 2 dogs, 20 mice, 12 rats, and 3 mongooses. Total DNA extracted from swab suspensions served as template for the detection of Y. enterocolitica DNA by real-time PCR. Neither Y. enterocolitica organisms nor their DNA were detected from any of these samples. However, Western blotting revealed the presence of Yersinia antibodies in plasma. The AGM samples revealed a seroprevalence of 91% for Yersinia spp. and of 61% for Y. enterocolitica specifically. The AGM that were housed in cages where at least one fatality occurred during the outbreak (clinical group) had similar seroprevalence to that of AGM housed in unaffected cages (nonclinical group). However, the nonclinical group was older than the clinical group. In addition, 25%, 100%, 33%, 10%, and 10% of the sampled local cats, dogs, mongooses, rats, and mice, respectively, were seropositive. The high seroprevalence after this outbreak suggests that Y. enterocolitica was transmitted effectively through the captive AGM population and that age was an important risk factor for disease. Knowledge regarding local environmental sources of Y. enterocolitica and the possible role of wildlife in the maintenance of yersiniosis is necessary to prevent and manage this disease.
机译：在NHP研究机构爆发小肠结肠炎耶尔森菌后，我们对耶尔森菌的流行进行了多物种调查。为了更好地了解耶尔森病的流行病学，在该设施中居住或觅食的各种哺乳动物中都可以找到它们。从105只圈养的非洲绿猴（AGM），12只野猫，2只狗，20只小鼠，12只大鼠和3只猫鼬获得了血液样本以及粪便和直肠拭子。从拭子悬液中提取的总DNA用作通过实时PCR检测小肠结肠炎耶尔森氏菌DNA的模板。从这些样品中均未检测到小肠结肠炎耶尔森氏菌及其DNA。然而，蛋白质印迹显示血浆中存在耶尔森氏菌抗体。 AGM样品显示耶尔森氏菌的血清阳性率为91％。对于小肠结肠炎耶尔森氏菌则为61％。暴发期间（临床组）发生至少一例死亡的笼中的AGM血清阳性率与未受影响的笼中（非临床组）的AGM相似。但是，非临床组比临床组大。此外，分别有25％，100％，33％，10％和10％的本地猫，狗，猫鼬，大鼠和小鼠是血清阳性的。此次暴发后的高血清阳性率表明，肠球菌耶氏杆菌通过圈养的AGM种群有效传播，而年龄是疾病的重要危险因素。有关预防和管理该病的必要知识是关于小肠结肠炎耶尔森氏菌的当地环境来源以及野生生物在维持耶尔森菌病中的可能作用。

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