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首页> 外文期刊>Biophysical Journal >Surfactant protein SP-B strongly modifies surface collapse of phospholipid vesicles: insights from a quartz crystal microbalance with dissipation.
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Surfactant protein SP-B strongly modifies surface collapse of phospholipid vesicles: insights from a quartz crystal microbalance with dissipation.

机译：表面活性剂蛋白SP-B强烈修饰磷脂囊泡的表面塌陷：石英晶体微天平的耗散见解。

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摘要

Pulmonary surfactant protein B (SP-B) facilitates the rapid transfer of phospholipids from bilayer stores into air-liquid interfacial films along the breathing cycle, and contributes to the formation of a surface-associated multilayer reservoir of surfactant to optimize the stability of the respiratory interface. To obtain more insights into the mechanisms underlying this transfer and multilayer formation, we established a simple model system that captures different features of SP-B action. We monitored the formation of supported planar bilayers from the collapse of intact phospholipid vesicles on a silica surface using a technique called quartz crystal microbalance with dissipation, which provides information on changes in membrane thickness and viscosity. At physiologically relevant concentrations, SP-B dramatically alters vesicle collapse. This manifests itself as a reduced buildup of intact vesicles on the surface before collapse, and allows the stepwise buildup of multilayered deposits. Accumulation of lipids in these multilayer deposits requires the presence of SP-B in both the receptor and the arriving membranes, surrounded by a comparable phospholipid charge. Thus, the quartz crystal microbalance with dissipation system provides a useful, simplified way to mimic the effect of surfactant protein on vesicle dynamics and permits a detailed characterization of the parameters governing reorganization of surfactant layers.
机译：肺表面活性剂蛋白B（SP-B）有助于沿呼吸循环将磷脂从双层存储迅速转移到气液界面膜中，并有助于形成表面相关的多层表面活性剂储集层，从而优化呼吸系统的稳定性接口。为了获得对这种转移和多层形成的潜在机制的更多了解，我们建立了一个简单的模型系统，该系统捕获了SP-B作用的不同特征。我们使用一种称为“消散”的石英晶体微天平技术，通过完整的磷脂囊泡在二氧化硅表面的塌陷来监控支撑的平面双层的形成，该技术可提供有关膜厚度和粘度变化的信息。在生理相关浓度下，SP-B会显着改变囊泡的塌陷。这表现为自身完整的囊泡在塌陷之前减少了堆积，并允许逐步堆积多层沉积物。这些多层沉积物中脂质的积累需要在受体和到达的膜中都存在SP-B，并被可比的磷脂电荷包围。因此，具有耗散系统的石英晶体微天平提供了一种有用的，简化的方法来模拟表面活性剂蛋白质对囊泡动力学的影响，并允许控制表面活性剂层重组的参数的详细表征。

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