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首页> 外文期刊>Comparative Medicine >Prevalence and Diagnosis of Hemotrophic Mycoplasma Infection in Research Sheep and Its Effects on Hematology Variables and Erythrocyte Membrane Fragility
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Prevalence and Diagnosis of Hemotrophic Mycoplasma Infection in Research Sheep and Its Effects on Hematology Variables and Erythrocyte Membrane Fragility

机译：绵羊血养性支原体感染的流行，诊断及其对血液学变量和红细胞膜脆性的影响

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摘要

Hemotrophic mycoplasma (hemoplasma) infection in research sheep can confound experimental results and contribute to morbidity and mortality. Prevalence and clinicopathologic studies historically relied on blood-smear diagnosis, but systematic studies using current molecular techniques are warranted. Here we sought to report the prevalence of subclinical infection in our study population, compare diagnostic sensitivity and specificity between blood smears and a PCR assay, and determine the effects of infection on CBC variables and erythrocyte membrane fragility. We collected whole-blood samples from 111 convenience-sampled research sheep. All samples were tested for hemoplasmas by using a PCR assay, blood smears were evaluated for visual presence of hemoplasmas, and CBC and osmotic fragility assays were performed. Subclinical prevalence, according to PCR diagnosis, was 14.1% (14 of 99) in our study population. Relative to the PCR assay, blood-smear diagnosis was 8.3% sensitive and 100% specific for hemoplasma detection. Subclinical infection was associated with changes in MCV, MCHC, RBC distribution width, and absolute monocyte count. Acute infection was associated with changes in RBC mass, Hgb concentration, MCV, MCH, MCHC, and absolute lymphocyte and monocyte counts. Acute infection was associated with increased mean erythrocyte fragility compared with that in uninfected control and treated sheep. We demonstrated that hemoplasma infection is common in our study population, blood-smear evaluation is insensitive at detecting infection, and infection is associated with changes in CBC variables and increased erythrocyte membrane fragility. These findings raise concerns regarding the suitability of hemoplasma-infected sheep for biomedical research.
机译：在研究绵羊中的贫血支原体（hemoplasma）感染会混淆实验结果，并助长发病率和死亡率。患病率和临床病理学研究历来都依赖于血涂片诊断，但是有必要使用当前的分子技术进行系统的研究。在这里，我们试图报告我们研究人群中亚临床感染的流行情况，比较血液涂片和PCR检测之间的诊断敏感性和特异性，并确定感染对CBC变量和红细胞膜脆性的影响。我们从111只方便采样的研究羊身上采集了全血样品。使用PCR测定法测试所有样品的血肿，评估血涂片是否可见血肿，并进行CBC和渗透脆性测定。根据PCR诊断，在我们研究的人群中，亚临床患病率为14.1％（99之14）。相对于PCR分析，血涂片诊断对血浆检测的敏感性为8.3％，特异性为100％。亚临床感染与MCV，MCHC，RBC分布宽度和绝对单核细胞计数的变化有关。急性感染与红细胞质量，Hgb浓度，MCV，MCH，MCHC以及绝对淋巴细胞和单核细胞计数的变化有关。与未感染的对照羊和治疗羊相比，急性感染与平均红细胞脆性增加有关。我们证明了血浆感染在我们的研究人群中很常见，血液涂片评估对检测感染不敏感，并且感染与CBC变量的变化和红细胞膜脆性的增加有关。这些发现引起了人们对感染血浆的绵羊是否适合生物医学研究的关注。

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