• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Comparative Medicine >Optimal age at fostering for derivation of Helicobacter hepaticus-free mice
【24h】

Optimal age at fostering for derivation of Helicobacter hepaticus-free mice

机译：培育无幽门螺杆菌小鼠的最佳年龄

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Helicobacter hepaticus is well established as an unwanted variable in laboratory rodent colonies. Historically, cesarean section and embryo transfer have been used to derive Helicobacter-free mouse colonies. Neonatal transfer of newborn mice onto Helicobacter-free foster dams was recently reported as an alternative method of deriving Helicobacter-free mice, but until now, the age by which pups must be fostered to remain Helicobacter-free was unknown. The purpose of the study reported here was to determine the age by which mouse pups must be fostered to remain free of H. hepaticus. Beginning on the day of birth, 20 C57BL/6 mice were fostered from H. hepaticus-positive parents onto Helicobacter-free BALB/c dams each day for 14 days for a total of 280 pups fostered. Fecal, specimens collected at weaning, and fecal, liver, and cecal specimens collected at euthanasia were analyzed by use of polymerase chain reaction (PCR) analysis. No pup fostered within 24 h of birth became infected with H. hepaticus; however, many of those fostered after 24 h became infected. These results were supported by those of a large field trial, in which 201 litters representing 71 strains of mice were fostered within 24 h of birth. Follow-up fecal PCR analysis was performed on 52 mice or their progeny that were randomly sampled from the 201 fostered litters. All mice tested remained free of H. hepaticus approximately 100 days after fostering. The results indicate that mouse pups must be fostered within 24 h of birth to remain free of H. hepaticus. In addition, cecal and fecal PCR analyses detected more infections, than did liver PCR analysis, thus indicating that those specimens are preferred for detection of H. hepaticus infection.
机译：众所周知，在实验室啮齿动物菌落中，肝幽门螺杆菌是一种不需要的变量。历史上，剖宫产和胚胎移植已被用于获得无幽门螺杆菌的小鼠菌落。最近有报道说，新生小鼠向无Helicobacter寄养坝的新生儿转移是获得Helicobacter无小鼠的另一种方法，但是直到现在，要养成幼崽以保持无Helicobacter的年龄尚不明确。此处报道的研究目的是确定必须培育小白鼠的年龄，以使其保持无肝杆菌的年龄。从出生当天开始，每天将20例C57BL / 6小鼠从肝炎性肝炎阳性父母中饲养到无幽门螺杆菌BALB / c母鼠上，共14天，共饲养280只幼崽。通过使用聚合酶链反应（PCR）分析来分析粪便，断奶时收集的标本以及安乐死时收集的粪便，肝脏和盲肠标本。出生后24小时内没有养成的幼犬感染了肝菌;但是，许多在24小时后寄养的人被感染。这些结果得到了一项大型田间试验的支持，该试验在出生24小时内培养了代表71个品系的201窝幼仔。对52只小鼠或其后代进行了后续粪便PCR分析，这些小鼠是从201只饲养的猫砂中随机取样的。在培养后约100天，所有测试的小鼠均保持无肝杆菌。结果表明，必须在出生后24小时内饲养小鼠幼崽，以使其保持无肝嗜血杆菌。另外，盲肠和粪便PCR分析比肝PCR分析检测到更多的感染，因此表明那些标本对于肝炎H.感染的检测是优选的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号