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首页> 外文期刊>Comparative Medicine >Effects of Route of Inoculation and Viral Genetic Variation on Antibody Responses to Polyomavirus SV40 in Syrian Golden Hamsters
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Effects of Route of Inoculation and Viral Genetic Variation on Antibody Responses to Polyomavirus SV40 in Syrian Golden Hamsters

机译：接种途径和病毒遗传变异对叙利亚金仓鼠多瘤病毒SV40抗体应答的影响

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Genetic variants of polyomavirus SV40 are powerful agents with which to define viral effects on cells and carcinogenesis pathways. We hypothesized that differences in biologic variation among viral strains affect the process of viral infection and are reflected in antibody responses to the viral nonstructural large T-antigen (TAg) protein but not in neutralizing antibody responses against the inoculated viral particles. We analyzed the production of TAg antibody and neutralizing antibody in Syrian golden hamsters that were inoculated with SV40 viral strains by intracardiac, intravenous, or intraperitoneal routes and remained tumor free. Compared with the intraperitoneal route, intravascular (that is, intravenous, intracardiac) inoculation resulted in increased frequency of responsiveness to TAg but not in higher TAg antibody titers. The intravascular route was superior both for eliciting neutralizing antibody responses and for higher titers of those responses. Viruses with complex regulatory regions induced TAg antibody more often than did viruses with simple regulatory regions after intraperitoneal but not intravascular injections, with no differences in antibody titers. This viral genetic variation had no effect on neutralizing antibody production after intraperitoneal or intravascular inoculations or on neutralizing antibody titers achieved. These findings confirm that SV40 variants differ in their biologic properties. Route of inoculation combined with viral genetic variation significantly influence the development of serum antibodies to SV40 TAg in tumor-free hamsters. Route of inoculation-but not viral genetic variation-is an important factor in production of neutralizing antibody to SV40.
机译：多瘤病毒SV40的遗传变异是强大的药物，可用来定义病毒对细胞和致癌途径的作用。我们假设病毒株之间的生物学差异的差异会影响病毒感染的过程，并反映在对病毒非结构性大T抗原（TAg）蛋白的抗体反应中，而不是在中和针对接种病毒颗粒的抗体反应中。我们分析了通过心脏内，静脉内或腹膜内途径接种SV40病毒株的叙利亚金仓鼠中TAg抗体和中和抗体的产生。与腹膜内途径相比，血管内（即静脉内，心脏内）接种导致对TAg的反应频率增加，但没有导致更高的TAg抗体滴度。血管内途径在引发中和抗体应答和这些应答的更高滴度方面都是优越的。腹膜内注射但血管内注射后，具有复杂调控区的病毒比带有简单调控区的病毒更容易诱导TAg抗体，抗体效价没有差异。该病毒遗传变异对腹膜内或血管内接种后中和抗体的产生或所达到的中和抗体效价没有影响。这些发现证实了SV40变体的生物学特性不同。接种途径与病毒遗传变异显着影响无瘤仓鼠中针对SV40 TAg的血清抗体的发育。接种途径而非病毒遗传变异是产生针对SV40的中和抗体的重要因素。

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