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首页> 外文期刊>Comparative Medicine >Subregional Expression of Hippocampal Glutamatergic and GABAergic Genes in F344 Rats with Social Isolation after Weaning
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Subregional Expression of Hippocampal Glutamatergic and GABAergic Genes in F344 Rats with Social Isolation after Weaning

机译：断奶后社交隔离的F344大鼠海马谷氨酸能和GABA能基因的亚区域表达

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Many studies have shown that postweaning social isolation (pwSI) alters various behavioral phenotypes, including hippocampus-dependent tasks. Here, we report the comprehensive analysis of the expression of glutamatergic and GABAergic neurotransmission related genes in the distinct hippocampal subregions of pwSI rats. Male F344 rats (age, 4 wk) experienced either pwSI or group housing (controls). At 7 wk of age, the hippocampus of each rat was removed and laser-microdissected into the CA1 and CA3 layers of pyramidal cells and the granule cell layer of the dentate gyrus. Subsequently, the expression of glutamatergic- and GABAergic-related genes was analyzed by quantitative RT-PCR. In the CA1 and CA3 pyramidal cell layers, 18 of 24 glutamate receptor subunit genes were at least 1.5-fold increased in expression after pwSI. In particular, the expression of several N-methyl-D-aspartate and kainate receptors (for example, Grin2a in CA1, Grik4 in CA3) was significantly increased after pwSI. In contrast, pwSI tended to decrease the expression of GABA(A) receptor subunit genes, and Gabra1, Gabra2, Gabra4, Gabra5, Gabrb2, Gabrg1, and Gabrg2 were all significantly decreased in expression compared with the levels in the group-housed rats. These results indicate a subregion specific increase of glutamate receptors and reduction of GABA(A) receptors, suggesting that the hippocampal circuits of pwSI rats may be in more excitable states than those of group-housed rats.
机译：许多研究表明，断奶后的社会隔离（pwSI）会改变各种行为表型，包括海马依赖性任务。在这里，我们报告了在pwSI大鼠的不同海马亚区中谷氨酸能和GABA能神经传递相关基因的表达的综合分析。雄性F344大鼠（年龄，4 wk）经历了pwSI或组住房（对照组）。在7周龄时，将每只大鼠的海马取出，并激光显微切割成锥体细胞的CA1和CA3层以及齿状回的颗粒细胞层。随后，通过定量RT-PCR分析了谷氨酸能和GABA能相关基因的表达。在CA1和CA3锥体细胞层中，pwSI后24个谷氨酸受体亚基基因中的18个表达至少增加了1.5倍。特别地，pwSI后，一些N-甲基-D-天冬氨酸和海藻酸酯受体（例如，CA1中的Grin2a，CA3中的Grik4）的表达显着增加。相比之下，pwSI倾向于降低GABA（A）受体亚基基因的表达，并且与组内大鼠的水平相比，Gabra1，Gabra2，Gabra4，Gabra5，Gabrb2，Gabrg1和Gabrg2的表达均显着降低。这些结果表明谷氨酸受体的亚区域特异性增加和GABA（A）受体的减少，表明pwSI大鼠的海马回路可能比成群饲养的大鼠更容易兴奋。

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