• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Comparative Medicine >Confounding influences on phenotype expression in MRL/lpr mice
【24h】

Confounding influences on phenotype expression in MRL/lpr mice

机译：混杂因素对MRL / lpr小鼠表型表达的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A colony of 47 four-month-old MRL/MpJ-Fas~(lpr) mice either heterozygous or wildtype for IL10 targeted mutation were reported to the Veterinary Clinical Service because of acute, bilateral, red, ocular discharge. They were scruffy with a diverse array of additional signs. All mice were lethargic, moderately dehydrated, and had multiple subcutaneous swellings on the face and neck. Several mice had multifocal, scaly, epidermal lesions, and the aforementioned discharge from one or both eyes. They were housed on corncob bedding in polycarbonate shoebox-type cages in groups of 4-5 per cage since procurement from Jackson Laboratories. Mice were serologically negative for Ectromelia virus (mouse pox), GDVII (Theiler's) virus, Lymphocytic choriomeningitis (LCM) virus, Mouse hepatitis virus (MHV), Mouse parvovirus (MPV), Mycoplasma pulmonis, Pneumonia virus of mice (PVM), Rotavirus (EDIM), and sendai virus. They were offered water ad libitum from bottles and fed a standard rodent chow diet. The mice were used to investigate the role of autoreactive T cells in the pathogenesis of systemic lupus erythematosus (SLE), which required collection of blood samples from the retro-orbital venous plexus every 4 weeks. Laboratory personnel had recently participated in a mandatory re-training seminar because of technical problems with repeated retro-orbital venipuncture. The colony was not exposed to any deleterious environmental factors or infectious agents. The published life span of MRL/MpJ-Fas~(lpr) mice is approximately 17 to 22 weeks; however, this colony was experiencing unexpected early morbidity and mortality. Unfortunately, the investigator discarded the affected mice before a diagnostic work-up could be performed.
机译：向兽医临床服务处报告了47只4个月大的杂合子或野生型IL10靶向突变的4个月大MRL / MpJ-Fas-（lpr）小鼠的菌落，因为其急性，双侧，红色，眼部放电。他们were着脚，上面有各种各样的附加标志。所有小鼠都嗜睡，中度脱水，并且在面部和颈部有多个皮下肿胀。几只小鼠患有多灶，鳞状，表皮损伤，并且一只或两只眼睛有上述放电。自从杰克逊实验室（Jackson Laboratories）采购以来，它们被安置在聚碳酸酯鞋盒型笼中的玉米芯床上，每笼4-5个一组。小鼠的血清学检查结果为：埃斯特罗菌病毒（小鼠痘），GDVII（泰勒氏）病毒，淋巴细胞性脉络膜脑膜炎（LCM）病毒，小鼠肝炎病毒（MHV），小鼠细小病毒（MPV），肺炎支原体，小鼠肺炎病毒（PVM），轮状病毒（EDIM）和仙台病毒。他们从瓶中随意喝水，并喂以标准的啮齿动物食物。小鼠用于研究自身反应性T细胞在系统性红斑狼疮（SLE）发病机理中的作用，该疾病每4周需要从眼眶后静脉丛收集血液样本。由于重复的眶后静脉穿刺术存在技术问题，实验室人员最近参加了强制性的再培训研讨会。该菌落没有暴露于任何有害的环境因素或传染原。已发表的MRL / MpJ-Fas〜（lpr）小鼠的寿命约为17至22周;但是，这个殖民地正经历着意想不到的早期发病率和死亡率。不幸的是，研究人员在进行诊断检查之前将患病的小鼠丢弃。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号