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首页> 外文期刊>Comparative Medicine >Use of frozen-thawed oocytes for efficient production of normal offspring from cryopreserved mouse spermatozoa showing low fertility
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Use of frozen-thawed oocytes for efficient production of normal offspring from cryopreserved mouse spermatozoa showing low fertility

机译：冻融的卵母细胞用于从低温保存的小鼠精子中有效繁殖低繁殖力的正常后代的用途

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摘要

Freezing of spermatozoa and unfertilized oocytes is a useful tool for the conservation of mouse genetic resources. However, the proportion of frozen-thawed oocytes fertilized with spermatozoa in vitro is low because spermatozoa, especially those frozen-thawed, can not penetrate into oocytes because of hardening of the zona pellucida following premature release of cortical granules. To produce offspring efficiently from cryopreserved transgenic mouse gametes, we fertilized frozen-thawed gametes by using intracytoplasmic sperm injection (ICSI) and assessed pre- and postimplantation development of embryos. Compared with fresh unfertilized oocytes, frozen-thawed unfertilized oocytes were highly tolerant to damage by injection, as the survival rates after injection of frozen spermatozoa were 51 and 78%, respectively. Frozen-thawed oocytes that survived after sperm injection developed normally to the blastocyst stage and gave rise to offspring. Moreover, offspring with transgenes also were obtained from frozen gametes fertilized by ICSI. These results demonstrate that ICSI is an efficient technique for producing offspring from transgenic spermatozoa showing low fertility and that use of frozen-thawed oocytes leads to conservation of genetic resources because suboptimally preserved gametes are not wasted.
机译：精子和未受精卵母细胞的冷冻是保存小鼠遗传资源的有用工具。然而，在体外受精后受精的卵子的比例很低，因为精子，特别是冻融的，由于过早释放皮质颗粒而使透明带变硬，因此不能渗透到卵母细胞中。为了从冷冻保存的转基因小鼠配子中有效产生后代，我们使用胞浆内的精子注射剂（ICSI）使冻融的配子受精，并评估了胚胎植入前和植入后的发育。与新鲜的未受精卵母细胞相比，冻融的未受精卵母细胞对注射损伤具有高度的耐受性，因为注射冷冻精子后的存活率分别为51％和78％。注射精子后存活的冻融卵母细胞正常发育至胚泡期并产生后代。此外，具有转基因的后代也从通过ICSI受精的冷冻配子中获得。这些结果表明，ICSI是一种从低生育力的转基因精子生产后代的有效技术，并且冷冻解冻的卵母细胞的使用导致遗传资源的保存，因为不会浪费亚最佳保存的配子。

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