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首页> 外文期刊>Comparative Medicine >7,12-Dimethylbenz[A]Anthracene Induces Sertoli-Leydig-Cell Tumors in the Follicle-Depleted Ovaries of Mice Treated with 4-Vinylcyclohexene Diepoxide.
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7,12-Dimethylbenz[A]Anthracene Induces Sertoli-Leydig-Cell Tumors in the Follicle-Depleted Ovaries of Mice Treated with 4-Vinylcyclohexene Diepoxide.

机译：7,12-二甲基苯并[A]蒽在用4-乙烯基环己烯二环氧化物处理的小鼠滤泡少的卵巢中诱导Sertoli-Leydig细胞肿瘤。

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摘要

Ovarian cancer is associated with high mortality due to its late onset of symptoms and lack of reliable screening methods for early detection. Furthermore, the incidence of ovarian cancer is higher in postmenopausal women. Mice rendered follicle-depleted through treatment with 4-vinylcyclohexene diepoxide (VCD) are a model of ovary-intact menopause. The present study was designed to induce ovarian neoplasia in this model by treating mice with 7,12-dimethylbenz[a]anthracene (DMBA). Female B6C3F1 mice (age, 28 d) received intraperitoneal sesame oil (vehicle; VCD- groups) as a control or VCD (160 mg/kg; VCD+ groups) daily for 20 d to cause ovarian failure. Four months after the onset of dosing, mice from each group received a single injection of DMBA (VCD-DMBA+ and VCD+DMBA+ groups, n = 15 per group) or vehicle control (VCD-DMBA-, n = 15; VCD+ DMBA-, n = 14) under the bursa of the right ovary. Ovaries were collected 3 or 5 mo after injection and processed for histologic evaluation. Immunohistochemistry was used to confirm classification of neoplasms. None of the animals in the VCD-DMBA- and VCD-DMBA+ groups (that is, mice still undergoing estrus) had tumors at either time point. At the 3-mo time point, 12.5% of the VCD+DMBA+ mice had ovarian tumors; at 5 mo, 57.1% of the VCD+DMBA+ and 14.3% of VCD+DMBA- ovaries had neoplasms. Neoplasms stained positively for inhibin l (granulosa cells) and negatively for keratin 7 (surface epithelium), thus confirming classification of the lesions as Sertoli-Leydig cell tumors. These findings provide evidence for an increased incidence of DMBA-induced ovarian neoplasms in the ovaries of follicle-depleted mice compared with that in age-matched cycling controls.
机译：卵巢癌由于症状发作晚，缺乏可靠的早期筛查方法，因此死亡率高。此外，绝经后妇女卵巢癌的发病率更高。通过用4-乙烯基环己烯二环氧化合物（VCD）处理而使卵泡耗尽的小鼠是卵巢完整绝经的模型。本研究旨在通过用7,12-二甲基苯并[a]蒽（DMBA）治疗小鼠在该模型中诱导卵巢肿瘤。雌性B6C3F1小鼠（年龄28天）每天接受腹膜内芝麻油（车辆; VCD-组）作为对照组，或每天VCD（160 mg / kg; VCD +组）引起卵巢功能衰竭。给药开始四个月后，每组小鼠接受DMBA（VCD-DMBA +和VCD + DMBA +组，每组n = 15）或溶媒对照（VCD-DMBA-，n = 15; VCD + DMBA- ，n = 14）在右侧卵巢的滑囊之下。注射后3或5个月收集卵巢并进行组织学评估。免疫组织化学用于确认肿瘤的分类。 VCD-DMBA-和VCD-DMBA +组中的任何动物（即仍处于发情期的小鼠）在任何一个时间点都没有肿瘤。在3个月的时间点，有12.5％的VCD + DMBA +小鼠患有卵巢肿瘤。在5个月时，VCD + DMBA +的57.1％和VCD + DMBA-的14.3％的卵巢有肿瘤。肿瘤对抑制素l（颗粒细胞）染色阳性，对角蛋白7（表面上皮细胞）染色阴性，因此确认了病变为Sertoli-Leydig细胞肿瘤的分类。这些发现提供了证据，与年龄匹配的自行车对照组相比，在滤泡耗尽的小鼠卵巢中DMBA诱导的卵巢肿瘤发生率增加。

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