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A novel titanium wound chamber for the study of wound infections in pigs

机译：用于研究猪伤口感染的新型钛伤口室

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In the face of emerging multidrug-resistant microbes, reliable animal models are needed to study potential new therapies in infected wounds. To this end, we implanted screw-top titanium chambers subdermally in full-thickness wounds on both flanks (n = 6 per flank) of 2 Goettinger minipigs. After 1 wk, chambers were inoculated with Staphylococcus aureus, Pseudomonas aeruginosa, or vehicle only. Throughout the study, wound fluid was harvested for quantitative bacterial cultures to monitor infection. Animals were followed for 4 wk, after which tissue biopsies were taken for histologic analysis and quantitative bacterial counts. The implanted titanium chambers were well tolerated by the pigs throughout the study. After inoculation of the chambers, wound infection was established and maintained for 14 d. Despite infection, no systemic effects were noted. Cross-contamination was negligible, compared with the vehicle-only control. After tissue ingrowth, each chamber creates a closed system that allows harvest of exudate or application of substances without loss of material from the chamber. Because 12 chambers are implanted in each pig, researchers have the opportunity to compare multiple treatment options (for example, antibiotics, antimicrobial peptides, gene therapy) in the same animal, with no interindividual variation. We conclude that the use of titanium chambers in pigs provides a reliable and reproducible in vivo model to investigate wound healing, wound infection, and treatment options.
机译：面对新兴的耐多药微生物，需要可靠的动物模型来研究感染伤口中潜在的新疗法。为此，我们在2只Goettinger小型猪的两个侧面（每侧n = 6）的全厚度伤口中皮下植入了螺钉顶部钛制腔室。 1周后，将金黄色葡萄球菌，铜绿假单胞菌或媒介物接种于室内。在整个研究过程中，收集伤口液用于定量细菌培养以监测感染。随访动物4周，然后进行组织活检以进行组织学分析和定量细菌计数。在整个研究过程中，猪对钛植入室的耐受性良好。接种室后，确定伤口感染并维持14 d。尽管有感染，但未发现全身作用。与纯车辆相比，交叉污染可以忽略不计。组织向内生长后，每个腔室将创建一个封闭系统，该系统可收集渗出液或施加物质，而不会损失腔室中的材料。因为每只猪都植入了12个隔室，所以研究人员有机会比较同一只动物的多种治疗选择（例如，抗生素，抗菌肽，基因疗法），并且没有个体差异。我们得出的结论是，在猪中使用钛制腔室可提供可靠且可重现的体内模型，以研究伤口愈合，伤口感染和治疗方案。

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