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首页> 外文期刊>Colloids and Surfaces, B. Biointerfaces >Nanoscale topography reduces fibroblast growth, focal adhesion size and migration-related gene expression on platinum surfaces
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Nanoscale topography reduces fibroblast growth, focal adhesion size and migration-related gene expression on platinum surfaces

机译：纳米形貌可减少成纤维细胞生长，粘着斑大小和铂表面上与迁移相关的基因表达

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摘要

Controlling cellular responses on biomaterial surfaces is crucial in biomedical applications such as tissue engineering and implantable prosthetics. Since cells encounter various nanoscale topographic features in their natural environment, it has been postulated that surface nanotopography may be an alternative route to fabricate biomaterials with a desirable cellular response. In this framework, we investigated the responses of primary human fibroblasts to platinum substrates with different levels of surface roughness at the nanoscale. The nanorough surfaces were fabricated by using the glancing angle deposition technique (GLAD). We found that levels of cellular responses depended on the surface roughness and the size of the nanoscale features. We showed that in response to nanotopography cells spread less and have an elongated morphology, displaying signs of actin cytoskeleton impairment and reduced formation of focal adhesion complexes. Although cell growth and adhesion were impaired on the nanorough substrates, cell viability was not affected by topography. To a minor extent our results also indicate that cell migration might be reduced on the nanorough surfaces, since a significantly lower gene expression of migration related genes were found on the roughest surfaces as compared to the flat reference. The results presented here demonstrate that surface nanotopography influences fibroblasts responses on platinum, which may be used to reduce cellular adhesion on platinum implant surfaces such as implantable neural electrodes.
机译：在生物医学应用（例如组织工程和植入式修复术）中，控制生物材料表面的细胞反应至关重要。由于细胞在其自然环境中会遇到各种纳米尺度的地形特征，因此推测表面纳米外形可能是制造具有所需细胞反应的生物材料的另一种途径。在此框架下，我们研究了原始人类成纤维细胞对纳米级不同表面粗糙度水平的铂底物的响应。纳米粗糙表面是通过使用掠角沉积技术（GLAD）制成的。我们发现细胞反应的水平取决于表面粗糙度和纳米级特征的大小。我们表明响应纳米形貌细胞传播较少，并具有拉长的形态，显示肌动蛋白细胞骨架受损的迹象，减少了黏着斑复合物的形成。尽管在纳米粗糙基底上细胞生长和粘附受到损害，但是细胞存活率不受地形影响。在较小程度上，我们的结果还表明，由于与平坦参照物相比，在最粗糙的表面上发现了与迁移相关的基因的明显更低的基因表达，因此在纳米粗糙表面上的细胞迁移可能会减少。此处显示的结果表明，表面纳米形貌会影响成纤维细胞对铂的反应，可用于减少铂植入物表面（如可植入神经电极）上的细胞粘附。

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