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首页> 外文期刊>Comparative Medicine >Lymphocytic choriomeningitis infection undetected by dirty-bedding sentinel monitoring and revealed after embryo transfer of an inbred strain derived from wild mice.
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Lymphocytic choriomeningitis infection undetected by dirty-bedding sentinel monitoring and revealed after embryo transfer of an inbred strain derived from wild mice.

机译：淋巴膜绒毛膜脑膜炎感染未通过肮脏的床上用品前哨监测发现，并在胚胎移植后衍生自野生小鼠的近交菌株后发现。

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摘要

Persistent LCMV infection in wild-derived MAI/Pas mice housed under conventional conditions remained undetected for a decade, despite periodic health monitoring using dirty-bedding sentinels. When MAI/Pas mice were rederived by embryo transfer, recipient mothers produced antiLCMV antibodies, which first revealed the presence of the virus in the colony. Before this information was obtained, MAI/Pas mice had been shipped to another facility, undergone cesarean rederivation there, and been introduced into the recipient barrier. The foster mothers of rederived pups were LCMV-negative according to enzyme-linked immunosorbent assay, but sera of both cesarean-rederived MAI/Pas mice and their foster mothers were positive for LCMV infection by immunofluorescent assay (IFA). LCMV was isolated from the MAI/Pas mice, and its genomic RNA was sequenced. Examination of animal technicians in contact with LCMV-infected mice and of other mouse samples by IFA or a reverse transcriptase-polymerase chain reaction test (or both) revealed that neither the workers nor other animals had been infected with LCMV. Experimental data showed that LCMV transmission from persistently infected mice to naive ones occurred only after direct contact of animals housed in the same cage. This experience demonstrates the importance of careful viral monitoring in the transfer of laboratory rodents between institutions, the limitation of dirty-bedding sentinels for detection of LCMV infection, and the inadequacy of cesarean rederivation for elimination of enzootic LCMV infection..
机译：在常规条件下饲养的野生MAI / Pas小鼠中，持久性LCMV感染十年来仍未发现，尽管使用肮脏的床上用品定期监测健康状况。当MAI / Pas小鼠通过胚胎移植而重新枯萎时，接受者的母亲产生了抗LCMV抗体，该抗体首先揭示了该病毒在菌落中的存在。在获得此信息之前，MAI / Pas小鼠已被运送到另一家医院，在那里进行了剖宫产，并被引入了受体屏障。根据酶联免疫吸附法，再流幼崽的寄养母亲LCMV阴性，但通过免疫荧光法（IFA）剖宫产再发MAI / Pas小鼠和他们的寄养母亲的血清均为LCMV阳性。从MAI / Pas小鼠中分离出LCMV，并对其基因组RNA进行了测序。通过IFA或逆转录酶-聚合酶链反应测试（或两者）对与LCMV感染小鼠和其他小鼠样品接触的动物技术人员进行检查，结果表明工人和其他动物均未感染LCMV。实验数据表明，LCMV从持续感染的小鼠传播到幼稚的小鼠仅在同一个笼子里的动物直接接触后才发生。这项经验表明，在机构之间的实验室啮齿动物转移中进行仔细的病毒监测，检测LCMV感染的肮脏床上用品的局限性以及剖宫产的重新适应性不足以消除动物源性LCMV感染的重要性。

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