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首页> 外文期刊>Colloids and Surfaces, B. Biointerfaces >Gradient immobilization of a cell adhesion RGD peptide on thermal responsive surface for regulating cell adhesion and detachment
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Gradient immobilization of a cell adhesion RGD peptide on thermal responsive surface for regulating cell adhesion and detachment

机译：将细胞粘附RGD肽梯度固定在热响应表面上，以调节细胞粘附和脱离

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摘要

Using surface initiated atomic transfer radical polymerization (ATRP) and an injection method, a poly(N-isopropylacrylamide)-b-poly(acrylic acid)-g-RGD (PNIPAAm-b-PAA-g-RGD) gradient surface was prepared. First, a thermoresponsive surface with a constant thickness of PNIPAAm was fabricated, onto which the AA monomers were block copolymerized using the PNIPAAm macromolecules as initiators. During this process, a continuous injection method was employed to yield a molecular weight gradient of PAA on the underlying uniform PNIPAAm layer. RGD peptide was finally covalently immobilized onto the PAA gradient by carbodiimide chemistry. In vitro culture of HepG2 cells showed that immobilization of the RGD peptide could accelerate cell attachment, while the thermoresponsive layer beneath could effectively release the cells by simply lowering temperature. Thus, the PNIPAAm-b-PAA-g-RGD gradient surface, combining the thermal response with cell affinity properties, can well regulate the cell adhesion and detachment, which may thus be useful for investigation of cell-substrate interactions with a smaller number of samples.
机译：使用表面引发的原子转移自由基聚合（ATRP）和注入方法，制备了聚（N-异丙基丙烯酰胺）-b-聚（丙烯酸）-g-RGD（PNIPAAm-b-PAA-g-RGD）梯度表面。首先，制造出具有恒定厚度PNIPAAm的热响应性表面，使用PNIPAAm大分子作为引发剂，将AA单体嵌段共聚。在此过程中，采用连续注入方法在下面的均匀PNIPAAm层上产生PAA的分子量梯度。最后，通过碳二亚胺化学将RGD肽共价固定在PAA梯度上。 HepG2细胞的体外培养显示，RGD肽的固定化可以加速细胞附着，而下方的热敏层可以通过简单地降低温度来有效释放细胞。因此，PNIPAAm-b-PAA-g-RGD梯度表面结合了热响应和细胞亲和力特性，可以很好地调节细胞的粘附和脱离，因此对于研究较少数量的细胞与底物之间的相互作用可能很有用。样品。

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