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首页> 外文期刊>Biopreservation and biobanking >Cryopreservation of Peripheral Blood Stem Cells Using a Box-in-Box Cooling Device
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Cryopreservation of Peripheral Blood Stem Cells Using a Box-in-Box Cooling Device

机译：使用盒中盒冷却设备冷冻保存外周血干细胞

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摘要

The cooling process is critical for the cryopreservation of human hematopoietic stem cells (HSCs). Currently, programmed freezing methods and uncontrolled cooling methods are in use, both having obvious disadvantages. In this article, a novel device termed Box-in-Box (BIB) was developed and evaluated by in vitro cryopreservation tests in 2 different operation modes ("against-side" mode for Group I (n = 10), and "in-middle" mode for Group II (n = 10), respectively), and compared with an uncontrolled cooling method (Group III (n = 7), Styrofoam boxes) as well as a conventional programmed freezer method (Group IV (n = 10), CryoMed TM 1010, Cryogenic Tech., FL). Recorded temperature profiles of samples cryopreserved with BIB show that a consistent cooling procedure with a rate around -1 degrees C to -3.5 degrees C/min can be achieved during their transfer from room temperature to an -80 degrees C freezer. Statistical analysis of the stem cell population recovery, survival, and colony generation recovery shows that there is no significant difference (P > 0.26) among the methods using the BIB or programmed freezer (Group I, Group II, and Group IV), and their related deviations are smaller than the uncontrolled cooling rate method (Group III). Methods using the BIB (Group I and Group II) generated significantly better cell survival rate (P < 0.01) than the uncontrolled cooling rate method (Group III). The results indicate that the controlled cooling rate methods (BIB or CryoMed PF) are more consistent and reliable for clinical use. Considering the advantages of low cost, durability, and no liquid nitrogen consumption for the cooling process, the BIB can be a good alternative to the programmed freezers for the cryopreservation of HSCs.
机译：冷却过程对于人类造血干细胞（HSC）的冷冻保存至关重要。当前，使用编程的冷冻方法和不受控制的冷却方法，两者均具有明显的缺点。在本文中，我们开发了一种新颖的设备，称为Box-in-Box（BIB），并通过体外冷冻保存测试以2种不同的操作模式进行了评估（第I组的“对抗”模式（n = 10），“ in- II组（n = 10）分别处于“中间”模式，并与不受控制的冷却方法（III组（n = 7），聚苯乙烯泡沫塑料盒）以及常规的程序冷冻方法（IV组（n = 10））进行比较，CryoMed TM 1010，Cryogenic Tech。，FL）。冷冻保存有BIB的样品的温度记录记录表明，在从室温转移到-80℃冷冻室的过程中，可以实现大约-1℃至-3.5℃/ min的恒定冷却程序。对干细胞种群恢复，存活和集落产生恢复的统计分析表明，使用BIB或程序冷冻机（组I，组II和组IV）及其方法之间的方法之间无显着差异（P> 0.26）相关偏差小于不受控制的冷却速率方法（第III组）。与未控制的冷却速率方法（III组）相比，使用BIB（I组和II组）的方法产生的细胞存活率（P <0.01）明显更好。结果表明，控制冷却速率方法（BIB或CryoMed PF）对于临床使用而言更加一致和可靠。考虑到低成本，耐用性和冷却过程无液氮消耗的优势，BIB可以很好地替代用于HSC冷冻保存的程序冷冻机。

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