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首页> 外文期刊>Biopreservation and biobanking >A Pilot Study of the ELFE Longitudinal Cohort: Feasibility and Preliminary Evaluation of Biological Collection
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A Pilot Study of the ELFE Longitudinal Cohort: Feasibility and Preliminary Evaluation of Biological Collection

机译：ELFE纵向队列的初步研究：生物学收集的可行性和初步评估

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Etude Longitudinale Franc?aise depuis l’Enfance (ELFE) will be a national French cohort of 20,000 children followed from birth to adulthood. Biological samples will be taken at birth to evaluate the fetal exposition to several substances. A pilot study was carried out in October 2007 to test the preanalytical factors that affected sample quality. A variety of fractions were collected by the midwife after delivery from different blood collection tubes. Options in the collection process were 2 daily transports of samples, centralized and standardized processing methodology, and storage of multiple aliquots in liquid nitrogen or at - 80. We analyzed preanalytical factors that could have affected coagulation and then soluble CD40 Ligand (sCD40L) as a quality control tool for serum quality. Cord blood and urine were collected from 82% and 84% of women, respectively, who agreed to be followed up in the ELFE project. The use of syringe was the main factor correlated with coagulation (relative risk: 2.79 [1.47; 5.31], P < 0.01). Maternity unit status was also associated with coagulation (RR: 1.48 [1.03; 2.13] in a private maternity unit vs. a public maternity) as well as time between collection and centrifugation (RR 1.03 [1; 1.07] when time between collection and centrifugation increases from 1 h). There were no extremely low sCD40L values indicating extreme exposures to room temperatures. This first evaluation study allowed us to stress the importance of carefully recording all potentially critical preanalytical variables that might be used at a large-scale level.
机译：法国全国练习曲练习队（ELFE）将成为法国全国性的队列，由出生到成年的20,000名儿童组成。出生时将采集生物样本以评估胎儿对几种物质的暴露。 2007年10月进行了一项试点研究，以测试影响样品质量的分析前因素。助产士从不同的采血管分娩后收集了各种馏分。收集过程中的选择是每天进行2次样品运输，集中和标准化的处理方法以及在液氮中或在-80下存储多个等分试样。我们分析了可能影响凝血的分析前因素，然后分析了可溶性CD40配体（sCD40L）作为血清质量的质量控制工具。分别从82％和84％的妇女那里收集了脐带血和尿液，他们同意在ELFE项目中进行随访。使用注射器是与凝血相关的主要因素（相对危险度：2.79 [1.47; 5.31]，P <0.01）。孕产单位的状态还与凝血（私人孕产单位与公共孕产单位的RR：1.48 [1.03; 2.13]）以及收集和离心分离之间的时间相关（RR 1.03 [1; 1.07]）。从1小时开始增加）。没有极低的sCD40L值，表明其极度暴露于室温下。首次评估研究使我们强调了认真记录所有可能在大规模使用的潜在关键分析前变量的重要性。

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