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首页> 外文期刊>Biopreservation and biobanking >Catalase prevents lipid peroxidation and enhances survival of caprine preantral follicles cryopreserved in a 1,2-propanediol-freezing medium
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Catalase prevents lipid peroxidation and enhances survival of caprine preantral follicles cryopreserved in a 1,2-propanediol-freezing medium

机译:过氧化氢酶可防止脂质过氧化,并提高冷冻在1,2-丙二醇冷冻培养基中的山羊窦前卵泡的存活率

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摘要

The objectives of this study were to determine: 1) the optimal concentration (1.0 or 1.5 M) and duration of exposure (5, 10, or 20 min) of ovarian tissue to 1,2-propanediol (PROH) on morphology and viability of caprine preantral follicles; and 2) the effect of supplementing cryopreservation medium supplementation with Trolox~? (0.1, 0.5, or 1.0 mM) or catalase (5, 10, or 20 IU/mL) on follicular morphology, viability, and lipid peroxidation. Cryopreservation decreased (p<0.05) percentages of normal follicles relative to the control (84%). Although supplementation of the cryopreservation medium (1.0 M PROH) with catalase (10 or 20 IU/mL) or Trolox? (0.1 mM) resulted in follicular morphology and viability similar to that in the controls (P>0.05), lipid peroxidation was reduced only when 20 IU/mL catalase was added to the cryopreservation medium.
机译:这项研究的目的是确定:1​​)卵巢组织的最佳浓度(1.0或1.5 M)和卵巢组织暴露于1,2-丙二醇(PROH)的持续时间(5、10或20分钟),以了解其形态和生存能力。山羊窦前卵泡; 2)补充Trolox〜?补充冷冻保存液的效果? (0.1、0.5或1.0 mM)或过氧化氢酶(5、10或20 IU / mL)的滤泡形态,生存能力和脂质过氧化作用。冷冻保存相对于对照(84%)降低了正常卵泡的百分比(p <0.05)。尽管用过氧化氢酶(10或20 IU / mL)或Trolox补充了冷冻保存培养基(1.0 M PROH)? (0.1 mM)的卵泡形态和活力与对照组相似(P> 0.05),仅当将20 IU / mL过氧化氢酶添加到冷冻保存培养基中时,脂质过氧化作用才会降低。

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