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首页> 外文期刊>Colloids and Surfaces, B. Biointerfaces >Potential application of polyisobutylene-polystyrene and a Lactobacillus protein to reduce the risk of device-associated urinary tract infections
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Potential application of polyisobutylene-polystyrene and a Lactobacillus protein to reduce the risk of device-associated urinary tract infections

机译：聚异丁烯-聚苯乙烯和乳杆菌蛋白的潜在应用可减少与设备相关的尿路感染的风险

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Urinary tract infections (UTI) associated with prostheses continue to cause significant morbidity despite the development and implementation of new biomaterials and device coatings. Ureteral stents, placed to improve drainage of the kidney can become a nidus for infection and potentially serious kidney damage, through attachment of pathogens and biofilm formation. The present study investigated two approaches to reducing the risk of ureteral stent infection. The first involved comparing the resistance to bacterial attachment of medical grade silicone rubber, the current gold standard biomaterial for urinary tract devices, to that of polyisobutylene-polystyrene block copolymer (PIB-PS), a polymer with proven biomaterial potential but as yet untested within the urinary tract. The second approach studied the capacity of a recombinant protein (p29) originally isolated from Lactobacillus fermentum RC-14 to inhibit bacterial attachment when used as a device coating. The protein coat was successfully identified by atomic force microscopy (AFM), gel electrophoresis and surface enhanced laser desorption/ionization (SELDI) protein chip analysis and shown to be present for at least 96 h. The coating and the PIB-PS surface alone were found to significantly reduce the attachment of two common uropathogenic species, Escherichia coli 67 and Enterococcus faecalis 1131 in vitro in the presence of phosphate buffered saline along. However, the effect was negated following additional urine coating of the sections prior to challenge, likely due to p29 desorption and the development of a urinary conditioning film. These findings highlight the complexity of translating in vitro data to the in vivo setting and the critical role urinary constituents play in the development of urinary tract device-associated infections.
机译：尽管开发和实施了新的生物材料和设备涂层，但与假体相关的尿路感染（UTI）仍继续引起严重的发病率。通过附着病原体和形成生物膜，可以改善肾脏引流的输尿管支架可能成为感染和潜在的严重肾脏损害的病灶。本研究调查了两种降低输尿管支架感染风险的方法。首先涉及将医用级硅橡胶（目前用于尿道器械的金标准生物材料）对细菌附着的抵抗力与聚异丁烯-聚苯乙烯嵌段共聚物（PIB-PS）的抵抗力进行比较，聚异丁烯-聚苯乙烯嵌段共聚物具有证明的生物材料潜力，但尚未在其中进行测试尿路。第二种方法研究了最初从发酵乳杆菌RC-14分离的重组蛋白（p29）在用作设备涂层时抑制细菌附着的能力。通过原子力显微镜（AFM），凝胶电泳和表面增强的激光解吸/电离（SELDI）蛋白芯片分析成功鉴定了蛋白外壳，并显示存在至少96小时。发现单独的涂层和PIB-PS表面可在存在磷酸盐缓冲盐水的情况下在体外显着减少两种常见的尿毒症菌种（大肠杆菌67和粪肠球菌1131）的附着。但是，在挑战前用额外的尿液包被这些部分后，效果可能消失，这可能是由于p29的解吸和尿液调节膜的形成。这些发现凸显了将体外数据转化为体内环境的复杂性，以及尿液成分在与尿路装置相关的感染发展中的关键作用。

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