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首页> 外文期刊>Clinical and experimental pharmacology & physiology >Reperfusion injury in the human forearm is mild and not attenuated by short-term ischaemic preconditioning.
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Reperfusion injury in the human forearm is mild and not attenuated by short-term ischaemic preconditioning.

机译：人前臂的再灌注损伤是轻度的，短期缺血预处理不会减轻这种损伤。

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SUMMARY 1. Ischaemia-reperfusion (IR) injury is an important contributor to tissue damage and has been shown to be attenuated by preconditioning (PC) in some animal models. A recent report has suggested that the forearm can be used for the study of this phenomenon in humans. We aimed to reproduce and further characterize this model. 2. Healthy young adult volunteers (mean (+/-SEM) age 32 +/- 6 years) were studied on two occasions. During one visit, IR alone was induced by 10 min of upper arm cuff occlusion, whereas on another occasion a PC stimulus (three 3 min cuff inflations) preceded IR. Endothelial function in the ischaemic arm was assessed by measuring arterial flow-mediated dilatation (FMD) and by calculation of forearm blood flow at baseline and 15 and 60 min after IR. Systemic venous blood was sampled from the non-ischaemic arm at baseline, after PC and at 2, 15 and 30 min after IR to assess neutrophil/leucocyte (CD11b) and platelet (bound glycoprotein IIb/IIIa and fibrinogen) activation, as well as numbers of platelet-leucocyte complexes, which were determined by flow cytometry. Because of a lack of measurable effects, the IR experiment was repeated with 20 min ischaemia in six subjects. 3. Five females and eight males completed the study. Flow-mediated dilatation was significantly impaired 30 min after IR (4.1 vs 6.2% at baseline; P < 0.05);however, this was not significantly attenuated by ischaemic PC (FMD reduction at 30 min compared with baseline was 2.1 +/- 0.5% with IR alone and 2.6 +/- 1.4% with IR after PC; NS). No significant effect was seen on the number of platelet-leucocyte aggregates or on white cell or platelet activation after IR alone or after IR with PC (P > 0.6 for all comparisons). Similar results were obtained in six subjects studied subjected to 20 min ischaemia. 4. In conclusion, in healthy young adults, brief periods of skeletal muscle ischaemia lead to arterial endothelial dysfunction, but no significant platelet or white cell activation. Preconditioning does not attenuate this effect on the endothelium. Further experiments with longer ischaemia times and varying PC stimuli may be necessary to produce measurable effects; however, this may prove difficult in conscious human subjects.
机译：概述1.缺血再灌注（IR）损伤是导致组织损伤的重要因素，并且在某些动物模型中已被预适应（PC）减轻。最近的一份报告表明，前臂可用于研究人类的这种现象。我们旨在复制并进一步表征该模型。 2.对健康的年轻成人志愿者（平均（+/- SEM）年龄32 +/- 6岁）进行了两次研究。在一次就诊中，上臂袖带闭塞10分钟可单独诱发IR，而在另一情况下，IR之前需进行PC刺激（三个3分钟的袖带充气）。通过测量动脉血流介导的扩张（FMD）并通过计算基线时以及IR后15和60分钟的前臂血流来评估缺血臂的内皮功能。在基线后，PC后，IR后第2、15和30分钟从非缺血性臂抽取全身静脉血，以评估中性粒细胞/白细胞（CD11b）和血小板（结合的糖蛋白IIb / IIIa和纤维蛋白原）的活化，以及通过流式细胞仪测定的血小板-白细胞复合物的数量。由于缺乏可测量的影响，因此对六名受试者进行了20分钟缺血的IR实验。 3.五名女性和八名男性完成了研究。 IR后30分钟血流介导的扩张明显受损（4.1相对于基线时的6.2％; P <0.05）;但是，缺血PC并没有显着减弱这一作用（与基线相比30分钟时FMD降低了2.1 +/- 0.5％单独使用IR，PC后使用IR则为2.6 +/- 1.4％; NS）。单独IR后或用PC进行IR后，对血小板-白细胞聚集体的数量或白细胞或血小板活化均未见明显影响（所有比较均P> 0.6）。在接受20分钟局部缺血治疗的六个受试者中获得了相似的结果。 4.总之，在健康的年轻人中，短暂的骨骼肌缺血可导致动脉内皮功能障碍，但没有明显的血小板或白细胞活化。预处理不会减弱对内皮的影响。为了产生可测量的效果，可能需要进行更长的缺血时间和不同的PC刺激实验。然而，这可能在有意识的人类受试者中证明是困难的。

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