...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biopreservation and biobanking >DogMATIC-A Remote Biospecimen Collection Kit for Biobanking
【24h】

DogMATIC-A Remote Biospecimen Collection Kit for Biobanking

机译：DogMATIC-A生物库远程生物样本采集套件

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Canine tumors are valuable comparative oncology models. This research was designed to create a sustainable biobank of canine mammary tumors for breast cancer research. The aim was to provide a well-characterized sample cohort for specimen sharing, data mining, and long-term research aims. Canine mammary tumors are most frequently managed at a local veterinary clinic or hospital. We adopted a biobank framework based on a large number of participating veterinary hospitals and clinics acting as collection centers that were serviced by a centralized storage facility. Recruitment was targeted at rural veterinary clinics. A tailored, stable collection kit (DogMATIC) was designed that was used by veterinarians in remote or rural locations to collect both fresh and fixed tissue for submission to the biobank. To validate this methodology the kit design, collection rate, and sample quality were analyzed. The Australian Veterinary Cancer Biobank was established as a network of 47 veterinary clinics and three veterinary pathology laboratories spanning over 200,000 km(2). In the first 12 months, 30 canine mammary tumor cases were submitted via the DogMATIC kit. Pure intact RNA was isolated in over 80% of samples with an average yield of 14.49 mu g. A large network biobank, utilizing off-site collection with the DogMATIC kit, was successfully coordinated. The creation of the Australian Veterinary Cancer Biobank has established a long-term, sustainable, comparative oncology research resource in Australia. There are broader implications for biobanking with this very different form of collection and banking.
机译：犬肿瘤是有价值的比较肿瘤学模型。这项研究旨在为乳腺癌研究创建犬乳腺肿瘤的可持续生物库。目的是为样本共享，数据挖掘和长期研究提供一个特征明确的样本队列。犬乳腺肿瘤最常见的是在当地的兽医诊所或医院进行治疗。我们采用了一个生物库框架，该框架建立在大量参与活动的兽医医院和诊所的基础上，这些医院和诊所是由集中存储设施提供服务的收集中心。招聘针对农村兽医诊所。设计了一种量身定制的，稳定的采集套件（DogMATIC），供偏远或农村地区的兽医使用，以采集新鲜和固定的组织以提交给生物库。为了验证该方法，对试剂盒设计，收集率和样品质量进行了分析。澳大利亚兽医生物库的建立是由47个兽医诊所和三个兽医病理实验室组成的网络，跨越200,000公里（2）。在头12个月中，通过DogMATIC试剂盒提交了30例犬乳腺肿瘤病例。在超过80％的样品中分离了纯净的完整RNA，平均产量为14.49μg。一个大型网络生物库，利用DogMATIC套件进行了异地采集，已成功进行了协调。澳大利亚兽医癌症生物库的创建已在澳大利亚建立了长期，可持续，比较肿瘤学的研究资源。通过这种截然不同的收集和银行形式，对生物银行产生了更广泛的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号