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首页> 外文期刊>Clinical and experimental pharmacology & physiology >Glomerular expression of endothelial nitric oxide synthase in deoxycorticosterone acetate-salt-treated rats.
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Glomerular expression of endothelial nitric oxide synthase in deoxycorticosterone acetate-salt-treated rats.

机译：内皮型一氧化氮合酶在脱氧皮质酮醋酸盐处理的大鼠中的肾小球表达。

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摘要

1. In the present study, we investigated the relationship between chronic volume loading and glomerular endothelial nitric oxide synthase (eNOS) expression. Immunohistochemical expression of eNOS in glomeruli was semiquantified by graded scores of staining intensity. Each glomerulus was isolated by microdissection and mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) in desoxycorticosterone acetate (DOCA)-salt-treated rats. 2. Glomerular expansion and dilatation of tubules were the main histological findings and glomerular and vascular injury scores were significantly higher in the DOCA-salt-treated group than in the control group. Endothelial NOS staining in glomeruli in DOCA-salt-treated animals was 81.4% lower than in control animals. Endothelial NOS mRNA was also detected at a very low rate in the kidney of treated rats compared with control rats (22/80 vs 74/80 glomeruli, respectively). 3. These results suggest that eNOS protein and mRNA expression in glomeruli was reduced by DOCA-salt loading. Chronic volume loading may damage the glomerulus and this may be mediated, at least in part, by disruption of eNOS.
机译：1.在本研究中，我们调查了慢性容量负荷与肾小球内皮一氧化氮合酶（eNOS）表达之间的关系。肾小球中eNOS的免疫组织化学表达通过染色强度分级评分进行半定量。通过显微解剖分离每个肾小球，并通过逆转录-聚合酶链反应（RT-PCR）在醋酸脱氧皮质酮（DOCA）-盐处理的大鼠中检测mRNA表达。 2.肾小球扩张和小管扩张是主要的组织学发现，DOCA盐治疗组的肾小球和血管损伤评分明显高于对照组。经DOCA盐处理的动物肾小球的内皮NOS染色比对照动物低81.4％。与对照大鼠相比，在治疗大鼠的肾脏中还发现内皮NOS mRNA的比率非常低（分别为22/80与74/80肾小球）。 3.这些结果表明，DOCA-盐加载降低了肾小球中eNOS蛋白和mRNA的表达。慢性容量负荷可能会损害肾小球，这可能至少部分地通过eNOS的破坏来介导。

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