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首页> 外文期刊>Biomacromolecules >A Blank Slate? Layer-by-Layer Deposition of Hyaluronic Acid and Chitosan onto Various Surfaces
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A Blank Slate? Layer-by-Layer Deposition of Hyaluronic Acid and Chitosan onto Various Surfaces

机译：空白板？透明质酸和壳聚糖在不同表面上的逐层沉积

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Although poly(a-hydroxy esters),especially the PLGA family of lactic acid/glycolic acid copolymers,have many properties which make them promising materials for tissue engineering,the inherent chemistry of surfaces made from these particular polymers is problematic.In vivo,they promote a strong foreign-body response as a result of nonspecific adsorption and denaturation of serum proteins,which generally results in the formation of a nonfunctional fibrous capsule.Surface modification post-production of the scaffolds is an often-utilized approach to solving this problem,conceptually allowing the formation of a scaffold with mechanical properties defined by the bulk material and molecular-level interactions defined by the modified surface properties.A promising concept is the so-called "blank slate": essentially a surface that is rendered resistant to nonspecific protein adsorption but can be readily activated to covalently bind bio-functional molecules such as extracellular matrix proteins,growth factors or polysaccharides.This study focuses on the use of the quartz crystal microbalance (QCM) to follow the layer-by-layer (LbL) electrostatic deposition of high molecular weight hyaluronic acid and chitosan onto PLGA surfaces rendered positively charged by aminolysis,to form a robust,protein-resistant coating.We further show that this surface may be further functionalized via the covalent attachment of collagen IV,which may then be used as a template for the self-assembly of basement membrane components from dilute Matrigel.The response of NIH-3T3 fibroblasts to these surfaces was also followed and shown to closely parallel the results observed in the QCM.
机译：尽管聚（α-羟基酯），尤其是乳酸/乙醇酸共聚物的PLGA系列具有许多特性，使其成为有希望用于组织工程的材料，但由这些特殊聚合物制成的表面的内在化学性质还是有问题的。由于血清蛋白的非特异性吸附和变性而促进强烈的异物反应，这通常导致形成无功能的纤维囊。支架生产后的表面改性是解决此问题的常用方法，从概念上讲，可以形成具有由大块材料定义的机械特性和由修饰的表面特性定义的分子水平相互作用的支架。一个有前途的概念是所谓的“空白板”：实质上是一种对非特异性蛋白质具有抗性的表面吸附，但可以很容易地激活以共价结合生物功能分子，例如细胞外基质蛋白s，生长因子或多糖。这项研究的重点是利用石英晶体微量天平（QCM）跟踪高分子量的透明质酸和壳聚糖逐层（LbL）静电沉积到通过氨解作用带正电的PLGA表面上我们进一步表明，该表面可通过胶原IV的共价连接进一步功能化，然后可将其用作自稀Matrigel的基底膜成分自组装的模板。还跟踪了NIH-3T3成纤维细胞对这些表面的反应，并显示出与QCM中观察到的结果非常相似。

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