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Solution Structure and Dynamics of Cartilage Aggrecan

机译:软骨蛋白聚糖的溶液结构和动力学

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We studied the structure and dynamics of porcine laryngeal aggrecan in solution using a range of noninvasive techniques:dynamic light scattering(DLS),small-angle neutron scattering(SANS),video particle tracking(VPT)microrheology,and diffusing wave spectroscopy(DWS).The data are analyzed within the framework of a combined static and dynamic scaling model,and evidence is found for reptation of the comb backbones with unentangled side-chain dynamics.Small-angle neutron scattering indicated standard polyelectrolyte scaling of the mesh size(ξ)with concentration(c)in semidilute solutions for the whole aggrecan aggregate,ξ = Ac~(-0.47±0.04),with the prefactor(A)implying there is on average 60 nm between the aggrecan subunits along the backbone.VPT demonstrated large exponents for the power law dependence of the intrinsic viscosity(η)on the polymer concentration in the semidilute concentration regime,η ~ c~α;with α equal to 2.04 ± 0.06 and 1.95 ± 0.08 for the assembled and disassembled aggrecan aggregates,respectively.DWS at high frequencies(10~4-10~5 Hz)gave evidence for internal Rouse modes of the aggrecan monomers,independent of the degree of self-assembly of the molecules.
机译:我们使用了一系列无创技术研究了猪喉头聚集蛋白聚糖在溶液中的结构和动力学:动态光散射(DLS),小角度中子散射(SANS),视频粒子跟踪(VPT)微流变学和扩散波谱(DWS)在静态和动态比例缩放模型的框架内对数据进行分析,并发现具有无纠缠侧链动力学的梳状骨架的证据。小角度中子散射表示标准聚电解质对网格尺寸的缩放(ξ)整个聚集蛋白聚糖聚集体在半稀释溶液中的浓度(c)为ξ= Ac〜(-0.47±0.04),而前因子(A)表示沿骨架的聚集蛋白聚糖亚基之间平均存在60 nm.VPT显示出大指数在半稀释浓度条件下,特性粘度(η)对聚合物浓度的幂定律依赖性;η〜c〜α;对于组装和拆卸的集料,α等于2.04±0.06和1.95±0.08 n分别聚集。DWS在高频率(10〜4-10〜5 Hz)下提供了聚集蛋白聚糖单体内部Rouse模式的证据,与分子的自组装程度无关。

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