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首页> 外文期刊>Clinical and laboratory haematology >Implementation of monoclonal antibody fluorescence on the Abbott CELL-DYN Sapphire haematology analyser: evaluation of lymphoid, myeloid and platelet markers.
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Implementation of monoclonal antibody fluorescence on the Abbott CELL-DYN Sapphire haematology analyser: evaluation of lymphoid, myeloid and platelet markers.

机译：在Abbott CELL-DYN蓝宝石血液学分析仪上实施单克隆抗体荧光：评估淋巴，骨髓和血小板标记。

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Apart from qualitative flags, that are typically inefficient and uninformative, haematology instruments provide little meaningful information about lymphocyte populations or the lineage of atypical or immature elements, The CELL-DYN Sapphire haematology analyser uses integrated optical and fluorescence (488 nm) measurements, with FL1 (FITC) and FL2 (PE) detectors being configured for fluorescent analysis. As monoclonal antibodies (Mab) are widely used as cellular probes, and are likely to constitute the future basis for immunodifferentials, we explored the feasibility of implementing immunofluorescence on this routine haematology analyser. An extensive series of Mab (CD2, CD3, CD4, CD8, CD11b, CD13, CD14, CD16, CD19, CD22, CD33, CD34, CD41, CD42b, CD45, CD56, CD61, CD64, CD235a and HLA-DR) were tested singly or in FITC/PE combinations. Analyser processing and data acquisition was achieved using CD-Sapphire automated CD61 immunoplatelet or CD3/4/8 assay procedures and, apart from mixing EDTA-blood and antibody, no further sample manipulation was required. Downloaded raw files were processed with cytometry software, and all evaluated reagents showed population discrimination analogous to flow cytometry. Practical procedures were straightforward and required minimal operator training. Extended information that can be obtained from monoclonal antibodies with a routine haematology analyser has the potential to extend haematology laboratory practices and positively impact laboratory and clinical efficiency.
机译：除了定性标记（通常效率低下和信息不足）外，血液学仪器几乎无法提供有关淋巴细胞数量或非典型或不成熟元素谱系的有意义的信息。CELL-DYN蓝宝石血液学分析仪使用集成的光学和荧光（488 nm）测量，以及FL1 （FITC）和FL2（PE）检测器配置用于荧光分析。由于单克隆抗体（Mab）被广泛用作细胞探针，并可能构成免疫鉴别的未来基础，因此，我们探索了在这种常规血液学分析仪上实施免疫荧光的可行性。测试了一系列广泛的Mab（CD2，CD3，CD4，CD8，CD11b，CD13，CD14，CD16，CD19，CD22，CD33，CD34，CD41，CD42b，CD45，CD56，CD61，CD64，CD235a和HLA-DR）单独或以FITC / PE组合形式使用。使用CD-Sapphire自动CD61免疫血小板或CD3 / 4/8分析程序可实现分析仪处理和数据采集，并且除了将EDTA血液和抗体混合外，无需其他样品处理。下载的原始文件使用流式细胞仪软件处理，所有评估的试剂均显示出与流式细胞仪相似的种群歧视。实际操作程序简单明了，只需最少的操作员培训。可以使用常规血液分析仪从单克隆抗体获得的扩展信息有可能扩展血液学实验室操作，并积极影响实验室和临床效率。

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