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首页> 外文期刊>Clinical and laboratory haematology >Microvolume fluorimetry for the determination of absolute CD4 and CD8 lymphocyte counts in patients with HIV: a comparative evaluation.
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Microvolume fluorimetry for the determination of absolute CD4 and CD8 lymphocyte counts in patients with HIV: a comparative evaluation.

机译：微量荧光法测定HIV患者CD4和CD8的绝对淋巴细胞计数：一项比较评估。

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摘要

This study compared CD4 and CD8 lymphocyte counts obtained by microvolume fluorimetry (MVF) with those derived by flow cytometry (FC). Samples from 192 patients with known or suspected HIV were analysed, and the distribution of CD4 counts for these samples ranged from 0 and 1,279/microl, with 142/192 (74%) of the samples having CD4 values of less than 400/microl. Good agreement between FC and MVF CD4 counts was found (MVF = 0.98 x FC + 7.30) although there was a minor constant inter-method bias of approximately +7 cells/microl for the MVF data. For CD8 counts there was a constant bias between the two methods of approximately +23 cells/microl for FC. Most outliers were associated with higher FC CD8 counts. Supplementary analyses showed a high level of agreement between FC and MVF methods for the CD4:CD8 ratios (MVF = 0.98 x FC). This suggests that observed discrepancies between FC and MVF methods were almost certainly a result of the influence of the absolute lymphocyte counts obtained from the haematology analyser. The results confirm that the IMAGN 2000 microvolume fluorimeter system can be used as an alternative to conventional flow cytometry for the enumeration of CD4 and CD8 counts.
机译：这项研究比较了微体积荧光法（MVF）和流式细胞术（FC）得出的CD4和CD8淋巴细胞计数。分析了来自192名已知或疑似HIV患者的样本，这些样本的CD4计数分布范围为0和1,279 / microl，其中142/192（74％）样本的CD4值小于400 / microl。尽管MVF数据存在较小的恒定的方法间偏差，大约为+7个细胞/微升，但FC和MVF CD4计数之间的一致性很好（MVF = 0.98 x FC + 7.30）。对于CD8计数，两种方法之间的FC偏差约为+23个细胞/微升。大多数异常值与较高的FC CD8计数有关。补充分析显示，对于CD4：CD8比率，FC和MVF方法之间的一致性很高（MVF = 0.98 x FC）。这表明观察到的FC和MVF方法之间的差异几乎可以肯定是血液学分析仪获得的绝对淋巴细胞计数影响的结果。结果证实，IMAGN 2000微体积荧光计系统可以用作常规流式细胞术的替代品，用于计数CD4和CD8计数。

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