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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Conversion for Avicel and AFEX pretreated corn stover by Clostridium thermocellum and simultaneous saccharification and fermentation: Insights into microbial conversion of pretreated cellulosic biomass
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Conversion for Avicel and AFEX pretreated corn stover by Clostridium thermocellum and simultaneous saccharification and fermentation: Insights into microbial conversion of pretreated cellulosic biomass

机译:热纤梭菌转化Avicel和AFEX预处理的玉米秸秆并同时糖化和发酵:对预处理的纤维素生物质进行微生物转化的见解

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摘要

In this study, efforts were taken to compare solubilization of Avicel and AFEX pretreated corn stover (AFEX CS) by SSF and Clostridium thermocellum fermentation, with an aim to gain insights into microbial conversion of pretreated cellulosic biomass. Solubilization rates for AFEX CS are comparable for the two systems while solubilization of Avicel is much faster by C. thermocellum. Initial catalyst loading impacts final cellulose conversion for SSF but not for C. thermocellum. Hydrolysis of the two substrates using cell-free C. thermocellum fermentation broth revealed much smaller difference in cellulose conversion than the difference observed for growing cultures. Tests on hemicellulose removal and particle size reduction for AFEX CS indicated that substrate accessibility is very important for enhanced solubilization by C. thermocellum.
机译:在这项研究中,我们努力比较了SSF和热纤梭菌发酵对Avicel和AFEX预处理的玉米秸秆(AFEX CS)的增溶作用,目的是深入了解预处理的纤维素生物质的微生物转化。 AFEX CS的增溶速率在两个系统中均相当,而热纤梭菌对Avicel的增溶速度要快得多。初始催化剂负载量会影响SSF的最终纤维素转化率,但不会影响热纤梭菌。使用无细胞热纤梭菌发酵液对两种底物的水解显示,纤维素转化率的差异远小于生长培养物中观察到的差异。 AFEX CS的半纤维素去除和粒径减小的测试表明,底物可及性对于增强热纤梭菌的增溶作用非常重要。

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