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首页> 外文期刊>Clinica chimica acta: International journal of clinical chemistry and applied molecular biology >Interference of deferasirox with assays for serum iron and serum unsaturated iron binding capacity during iron chelating therapy.
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Interference of deferasirox with assays for serum iron and serum unsaturated iron binding capacity during iron chelating therapy.

机译：地拉罗司干扰铁螯合治疗过程中血清铁和血清不饱和铁结合能力的测定。

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BACKGROUND: Deferasirox (DFX) is an oral iron chelator that is used worldwide for the treatment of iron overload. Although serum ferritin level is usually measured as a marker of the efficacy of DFX, we sometimes experienced unexplainable changes in other serum markers for iron. We hypothesized that photometric assays for serum iron (sFe) and unsaturated iron binding capacity (UIBC) might be affected by DFX. METHODS: Measurement of sFe and UIBC was performed using 4 different assay systems. The samples were prepared by adding 0-300 muM DFX to pooled human serum or 15 randomized human serum samples. In some experiments, DFX-iron complex (DFX-Fe) was prepared by mixing iron ammonium citrate solution and DFX solution. RESULTS: Measurement of sFe was influenced by DFX-Fe, while iron-free DFX showed no effect on the value of sFe; DFX-Fe was measured as sFe, undistinguishable from transferrin-bound iron. On the other hand, measurement of serum UIBC was influenced by DFX itself; DFX might have been bound to iron in the reagent used for the assay, leading to an increase in UIBC values. CONCLUSIONS: DFX affected the sFe and UIBC assay systems. We must be careful in observing these markers during iron chelation therapy with DFX.
机译：背景：Deferasirox（DFX）是一种口服铁螯合剂，在世界范围内用于治疗铁超负荷。尽管通常将血清铁蛋白水平作为DFX功效的指标，但有时我们在其他铁血清指标中遇到无法解释的变化。我们假设血清铁（sFe）和不饱和铁结合能力（UIBC）的光度测定法可能会受到DFX的影响。方法：使用4种不同的测定系统进行sFe和UIBC的测量。通过将0-300μMDFX添加到合并的人血清或15个随机人血清样品中来制备样品。在一些实验中，通过混合柠檬酸铁铵溶液和DFX溶液来制备DFX-铁络合物（DFX-Fe）。结果：DFX-Fe影响sFe的测定，而无铁DFX对sFe值无影响。 DFX-Fe的测量值为sFe，与运铁蛋白结合的铁没有区别。另一方面，血清UIBC的测定受DFX本身的影响。 DFX可能已绑定到用于测定的试剂中的铁，导致UIBC值增加。结论：DFX影响了sFe和UIBC测定系统。在用DFX进行铁螯合治疗期间，我们必须小心观察这些标记。

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