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首页> 外文期刊>Физиология растений >Cloning and Expression Analysis of a CMS-Related Gene BcCoil from Brassica campestris ssp. chinensis1
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Cloning and Expression Analysis of a CMS-Related Gene BcCoil from Brassica campestris ssp. chinensis1

机译：甘蓝型油菜CMS相关基因BcCoil的克隆与表达分析。中华1

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摘要

BcCoi.l, a cytoplasmic male sterility related gene, which was isolated from flower buds of Brassica campestris ssp. chinensis Makino using the RACE technology, was characterized and submitted to the NCBI GenBank (accession no. GU263836). The gene encodes a 67.78-kD protein containing 16 leucine-rich repeats and an N -terminal F-box motif and is extremely similar to Arabidopsis thaliana Coil gene. The Southern blot showed that BcCoil belongs to a multigene family. In A thaliana, the Coil gene is involved in jasmonate signaling, and Coil mutant displayed male sterility. In this study, qPCR results demonstrated that BcCoil was accumulated in stamens and was significantly higher expressed in flower organs of the maintainer line than in the CMS one. At microsporocyte development stage, the gene was expressed at a significantly lower extent in the CMS line than in the maintainer line. This expression profile presumes that BcCoil plays a role in early microspore development in non-heading Chinese cabbage.
机译：BcCoi.1是一种与细胞质雄性不育有关的基因，该基因是从芸苔属植物的花蕾中分离得到的。使用RACE技术对中华牧羊犬进行了表征，并提交给NCBI GenBank（登录号GU263836）。该基因编码一个67.78-kD蛋白，该蛋白含有16个富含亮氨酸的重复序列和一个N末端F-box基序，与拟南芥Coil基因极为相似。 Southern印迹表明，BcCoil属于多基因家族。在拟南芥中，Coil基因参与了茉莉酸酯信号传导，而Coil突变体表现出雄性不育。在这项研究中，qPCR结果表明，BcCoil积累在雄蕊中，在维持系的花器官中的表达显着高于CMS之一。在微孢子细胞发育阶段，该基因在CMS系中的表达水平明显低于维持系。该表达谱推测BcCoil在非抽穗大白菜的早期小孢子发育中起作用。

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