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首页> 外文期刊>Comptes rendus. Biologies >Desiccation tolerance of Hymenophyllacea filmy ferns is mediated by constitutive and non-inducible cellular mechanisms
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Desiccation tolerance of Hymenophyllacea filmy ferns is mediated by constitutive and non-inducible cellular mechanisms

机译：膜叶膜蕨的干燥耐性是由组成型和非诱导性细胞机制介导的

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The Hymenophyllaceae is a primitive family within the Filicopsidae. One of the most exceptional features of this family of ferns is the presence of fronds with one or just a few cell layers (hence their name of filmy ferns), and the absence of stomata. Hymenophyllum caudiculatum and Hymenophyllum dentatum are able to lose more than 82% of their fully hydrated water content, to remain dry for extended periods of time (days or weeks), and to survive and remain viable following rehydration. The aim of this work was to understand whether the adaptive strategy of the Hymenophyllaceae for desiccation tolerance is constitutive or inducible. A proteomic approach was adopted in combination with physiological parameters to assess whether there were changes in the protein content during dehydration and following rehydration. Detached fronds were used to monitor the rates of photosynthesis in desiccation experiments, sugar accumulation, and high-resolution 2-DE to analyze proteome variation during a desiccation rehydration cycle. The analyzed proteome exhibited little variation (3-4%) between hydrated and desiccated states, while variation was greater between the desiccated and rehydrated states (8.7 -10%). Eighty-two discrete proteins were analyzed by MS/MS, and 65 were identified. About 21% of the analyzed proteins (17) were mixtures of two or more different polypeptides. Of the identified proteins, more than a half (33 spots, 55%) had functions related to energyphotosynthesis. The second largest category with known function (five spots, 8%) was related to cell rescue, defense, and virulence. More than one in every four proteins analyzed belonged to a group of hypothetical proteins (18 spots, 28%). The results suggest that the Hymenophyllaceae represent an example of a change in adaptive strategy from a typical vascular to the poikilohydric homoiochlorophyllous adaptation, which they share with the bryophytes that grow in profusion in the same habitats. The speed at which desiccation takes place therefore precludes the induction of protective systems, suggesting a constitutive mechanism of cellular protection
机译：处女膜科是Filicopsidae内的原始家族。该蕨类家族最突出的特征之一是存在具有一层或几层细胞层的叶状体（因此得名薄膜状蕨类植物），并且没有气孔。沙门氏菌和齿状叶菌能够损失超过82％的完全水合水分，长时间保持干燥（几天或几周），并在复水后存活并保持活力。这项工作的目的是了解为干燥耐性的处女膜的适应性策略是本构还是可诱导的。结合蛋白质组学方法结合生理参数来评估脱水和脱水后蛋白质含量是否发生变化。分离的叶状体用于监测脱水实验中的光合作用速率，糖的积累和高分辨率的2-DE，以分析脱水复水周期中蛋白质组的变化。所分析的蛋白质组在水合状态和干燥状态之间的变化很小（3-4％），而在水合状态和复水状态之间的变化更大（8.7 -10％）。通过MS / MS分析了82种离散蛋白，鉴定出65种。约21％的分析蛋白质（17）是两种或多种不同多肽的混合物。在鉴定出的蛋白质中，超过一半（33个斑点，55％）具有与能量光合作用相关的功能。具有已知功能的第二大类别（五个点，占8％）与细胞拯救，防御和毒力有关。每四个分析的蛋白质中有一个以上属于一组假设的蛋白质（18个斑点，占28％）。结果表明，膜状叶菌科是适应策略从典型的脉管植物转变为多叶高纯叶绿素适应性变化的一个例子，它们与在相同生境中大量生长的苔藓植物共享。因此，发生干燥的速度排除了保护系统的诱导，提示了细胞保护的本构机制

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