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首页> 外文期刊>Computer Methods and Programs in Biomedicine: An International Journal Devoted to the Development, Implementation and Exchange of Computing Methodology and Software Systems in Biomedical Research and Medical Practice >Automation of the use of fluorescent microspheres for the determination of blood flow.
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Automation of the use of fluorescent microspheres for the determination of blood flow.

机译：自动使用荧光微球测定血流量。

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摘要

Fluorescent-labeled microspheres (FM) are a new tool for the determination of organ blood flow. However, the FM-method is labor intensive, because of the necessity to recover the microspheres from the tissue samples. The aim of this study was to automate the FM-method. A Zymate-Robotic System (Zymark, Idstein, Germany) was modified to handle a novel filtration device. The robot is surrounded by 12 different stations which are necessary to process the samples. It performs the sequential steps which are needed to recover the microspheres from the samples. The dyes are finally released from the FM with a solvent and their fluorescent intensity is measured online using a spectrophotometer (Perkin Elmer, Uberlingen, Germany). The robotic system is able to recover the FM through digestion and filtration of the tissue samples using the new filter, to dissolve the FM and to release the dyes so that their fluorescent intensities can be measured for the calculation of organ blood flow.
机译：荧光标记的微球（FM）是测定器官血流的新工具。但是，由于需要从组织样本中回收微球，因此FM方法是劳动密集型的。这项研究的目的是使FM方法自动化。 Zymate-Robotic系统（Zymark，Idstein，德国）进行了改装，以处理新型过滤装置。机器人周围有12个不同的工作站，这些工作站是处理样品所必需的。它执行从样品中回收微球所需的顺序步骤。最终，染料用溶剂从FM中释放出来，并使用分光光度计（Perkin Elmer，Uberlingen，德国）在线测量其荧光强度。机器人系统能够通过使用新的过滤器对组织样本进行消化和过滤来恢复FM，以溶解FM并释放出染料，从而可以测量其荧光强度来计算器官血流量。

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