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首页> 外文期刊>Computer Methods and Programs in Biomedicine: An International Journal Devoted to the Development, Implementation and Exchange of Computing Methodology and Software Systems in Biomedical Research and Medical Practice >Computer program for determining fluorescence resonance energy transfer efficiency from flow cytometric data on a cell-by-cell basis.
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Computer program for determining fluorescence resonance energy transfer efficiency from flow cytometric data on a cell-by-cell basis.

机译：用于基于流式细胞仪数据逐个细胞确定荧光共振能量转移效率的计算机程序。

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摘要

The determination of fluorescence resonance energy transfer (FRET) with flow cytometry (FCET) is one of the most efficient tools to study the proximity relationships of cell membrane components in cell populations on a cell-by-cell basis. Because of the high amount of data and the relatively tedious calculations, this procedure should be assisted by powerful data processing software. The currently available programs are not able to fulfill this requirement. We developed a Windows-based program to calculate fluorescence resonance energy transfer efficiency values from list mode flow cytometry standard (FCS) files. This program displays the measured data in standard plots by generating one- and two-parameter histograms on linear or logarithmic scales. A graphical gating tool allows the user to select the desired cell population according to any combination of the parameter values. The program performs several statistical calculations, including mean, S.D., percent of the gated data. We have implemented two types of data sheet for FRET calculations to aid and guide the user during the analysis: one with population-mean-based autofluorescence correction and the other with spectrum-based cell-by-cell autofluorescence correction. In this paper, we describe the gating algorithms, the file opening procedure and the rules of gating. The structure of the program and a short description of the graphical user-interface (GUI) are also presented in this article.
机译：用流式细胞仪（FCET）测定荧光共振能量转移（FRET）是研究逐细胞基础上细胞群体中细胞膜成分的邻近关系的最有效工具之一。由于大量的数据和相对繁琐的计算，因此应该借助功能强大的数据处理软件来辅助此过程。当前可用的程序无法满足此要求。我们开发了一个基于Windows的程序，用于从列表模式流式细胞术标准（FCS）文件计算荧光共振能量转移效率值。该程序通过生成线性或对数刻度的一参数和二参数直方图，在标准图中显示测量数据。图形选通工具允许用户根据参数值的任意组合选择所需的细胞群。该程序执行若干统计计算，包括门控数据的平均值S.D.。我们为FRET计算实现了两种数据表，以在分析过程中帮助和指导用户：一种通过基于群体均值的自发荧光校正，另一种通过基于光谱的逐个细胞自发荧光校正。在本文中，我们描述了选通算法，文件打开过程和选通规则。本文还介绍了程序的结构和图形用户界面（GUI）的简短说明。

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