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首页> 外文期刊>Clinical and experimental allergy : >Heterogeneity of commercial timothy grass pollen extracts.
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Heterogeneity of commercial timothy grass pollen extracts.

机译：商业提摩太草花粉提取物的异质性。

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BACKGROUND: The diagnosis and specific immunotherapy of allergy is currently performed with allergen extracts prepared from natural allergen sources. OBJECTIVE: To analyse commercial timothy grass pollen allergen extracts used for in vivo diagnosis regarding their qualitative and quantitative allergen composition and in vivo biological activity. METHODS: Antibodies specific for eight timothy grass pollen allergens (Phl p 1, Phl p 2, Phl p 4, Phl p 5, Phl p 6, Phl p 7, Phl p 12, Phl p 13) were used to detect these allergens in timothy grass pollen extracts from four manufacturers by immunoblotting. ELISA assays were developed and used to quantify the three major allergens (Phl p 1, Phl p 2, Phl p 5) in the extracts. The magnitude of skin responses to the four extracts was studied by skin prick testing in 10 grass pollen-allergic patients. RESULTS: The allergen extracts showed broad variations in protein compositions and amounts (24.1-197.7 microg/mL extract). Several allergens could not be detected in certain extracts or appeared degraded. A considerable variability regarding the contents of major allergens was found (Phl p 1: 32-384 ng/mL; Phl p 2: 1128-6530 ng/mL, Phl p 5: 40-793 ng/mL). Heterogeneous skin test results were obtained with the extracts in grass pollen-allergic patients. CONCLUSIONS: Timothy grass pollen extracts from different manufacturers exhibit a considerable heterogeneity regarding the presence of individual allergens and hence yield varying in vivo test results. Problems related to the use of natural grass pollen allergen extracts may be circumvented by using defined recombinant grass pollen allergens.
机译：背景：目前，过敏症的诊断和特异性免疫疗法是使用从天然过敏原来源制备的过敏原提取物进行的。目的：分析用于体内诊断的商业提摩太草花粉过敏原提取物的定性和定量过敏原组成以及体内生物学活性。方法：使用对八种提摩太草花粉过敏原（Phl p 1，Phl p 2，Phl p 4，Phl p 5，Phl p 6，Phl p 7，Phl p 12，Phl p 13）具有特异性的抗体来检测这些过敏原。通过免疫印迹从四个生产商的蒂莫西草粉花粉提取物。开发了ELISA测定法，并用于定量提取物中的三种主要变应原（Phl p 1，Phl p 2，Phl p 5）。通过皮肤点刺试验在10名草粉花粉过敏患者中研究了皮肤对这四种提取物的反应程度。结果：过敏原提取物在蛋白质组成和含量上有很大差异（24.1-197.7 microg / mL提取物）。在某些提取物中无法检测到几种过敏原，或者它们似乎已降解。发现关于主要过敏原含量的相当大的可变性（Phl p 1：32-384 ng / mL; Phl p 2：1128-6530 ng / mL，Phl p 5：40-793 ng / mL）。用草提取物对花粉过敏的患者的提取物获得了异质皮肤测试结果。结论：关于不同过敏原的存在，来自不同制造商的蒂莫西草花粉提取物表现出相当大的异质性，因此体内测试结果会有所不同。与天然草花粉过敏原提取物的使用有关的问题可以通过使用确定的重组草花粉过敏原来解决。

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