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首页> 外文期刊>Clinical and experimental allergy : >Lactic acid bacteria differ in their ability to induce functional regulatory T cells in humans.
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Lactic acid bacteria differ in their ability to induce functional regulatory T cells in humans.

机译：乳酸菌诱导人类功能性调节性T细胞的能力不同。

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BACKGROUND: Trials with probiotic lactic acid bacteria have yielded different results, which may be due to the strains used. Lactobacilli and bifidobacteria are known to be potent modulators of the immune system. The capacity of these bacteria used as probiotics to influence both T helper type 1 (Th1)- and Th2-mediated diseases has been shown before. However, the ability of strains to induce forkhead box P3 (FOXP3(+)) expressing regulatory T cells has not yet been investigated. OBJECTIVE: Test the inherent differences between strains in their capacity to induce functional regulatory T cells in human peripheral blood mononuclear cells (PBMC). METHODS: Human PBMC were co-cultured in vitro with either Bifidobacterium lactis W51, Lactobacillus acidophilus W55 or Lactobacillus plantarum W62 or an Escherichia coli control strain. The percentage of FOXP3(+) cells, the origin of the induced cells and the functionality of these cells were assessed. Results Probiotic strains differ in their capacity to induce regulatory T cells. FOXP3(+) cells were induced from CD25(-) cells and were able to suppress effector T cells. Naturally occurring regulatory T cells were not affected by co-culture with lactobacilli. IL-10 concentrations found in the supernatant showed a trend towards the same differences between strains. Blockade of IL-10 did not influence the up-regulation of FOXP3. No differences between lactic acid bacteria were found in IL-17, IFN-gamma or IL-13. CONCLUSIONS: Some probiotic strains are potent inducers of regulatory cells, while others are not. The clear differences between strains imply that an in vitro characterization of probiotic strains before application is recommended.
机译：背景：益生菌乳酸菌的试验产生了不同的结果，这可能是由于所使用的菌株引起的。已知乳酸杆菌和双歧杆菌是免疫系统的有效调节剂。以前已经证明了这些细菌作为益生菌影响T型辅助1型（Th1）和Th2介导的疾病的能力。但是，菌株诱导叉头盒P3（FOXP3（+））表达调节性T细胞的能力尚未进行调查。目的：测试菌株之间在人类外周血单核细胞（PBMC）中诱导功能性调节性T细胞的能力之间的固有差异。方法：将人PBMC与乳酸双歧杆菌W51，嗜酸乳杆菌W55或植物乳杆菌W62或大肠杆菌对照菌株进行体外共培养。评估FOXP3（+）细胞的百分比，诱导细胞的起源和这些细胞的功能。结果益生菌菌株诱导调节性T细胞的能力不同。从CD25（-）细胞诱导FOXP3（+）细胞，并能够抑制效应T细胞。天然存在的调节性T细胞不受乳杆菌共培养的影响。在上清液中发现的IL-10浓度显示了菌株之间相同差异的趋势。 IL-10的阻滞不影响FOXP3的上调。在IL-17，IFN-γ或IL-13中未发现乳酸菌之间的差异。结论：某些益生菌菌株是调节细胞的有效诱导剂，而另一些则不是。菌株之间的明显差异意味着建议在应用前对益生菌菌株进行体外鉴定。

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